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![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] - Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-EEF2K (Ser-78) (NBP3-23304) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23304_rabbit-eef2k-p-ser78-pab-19920231992815.jpg "Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] - Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-EEF2K (Ser-78) (NBP3-23304) at 1:1000.")
![Western Blot: BID [p Ser78] Antibody [NB500-260] - Detection of Mouse Phospho-BID (S78) after Treatment with EPE. Whole cell lysate (50 and 80 ug for WB; 1 mg for IP, 20% of IP loaded) from NIH3T3 cells that were treated with etoposide (100 mcM; 3 hours) or mock treated. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/BID-[p-Ser78]-Antibody-Western-Blot-NB500-260-img0004.jpg "Western Blot: BID [p Ser78] Antibody [NB500-260] - Detection of Mouse Phospho-BID (S78) after Treatment with EPE. Whole cell lysate (50 and 80 ug for WB; 1 mg for IP, 20% of IP loaded) from NIH3T3 cells that were treated with etoposide (100 mcM; 3 hours) or mock treated. Antibody used at 1 ug/ml.")
![Western Blot: Hsp27 Antibody (JBW502) [NBP2-29821] - Untreated (lane 1) and heat shock and arsenite treated (lane 2) MCF-7 cell lysate was probed with 0.05ug/ml of anti-phospho-HSP27 (Ser 78). Arrow indicates phosphorylated HSP27.](http://images.novusbio.com/fullsize/Hsp27-Antibody-JBW502-Western-Blot-NBP2-29821-img0001.jpg "Western Blot: Hsp27 Antibody (JBW502) [NBP2-29821] - Untreated (lane 1) and heat shock and arsenite treated (lane 2) MCF-7 cell lysate was probed with 0.05ug/ml of anti-phospho-HSP27 (Ser 78). Arrow indicates phosphorylated HSP27.")
![ELISA: Human HSP27 [p Ser78] ELISA Kit (Colorimetric) [NBP2-62152] - Standard Reference Curve](http://images.novusbio.com/fullsize/Human-HSP27-p-Ser78-ELISA-Kit-Colorimetric-ELISA-NBP2-62152-img0001.jpg "ELISA: Human HSP27 [p Ser78] ELISA Kit (Colorimetric) [NBP2-62152] - Standard Reference Curve")