Hu,
Mu,
Rt,
Po,
Dr,
Gt,
Ma,
Pm,
Pm,
Rb,
SyHaSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Dr, Gt, Ma, Pm, Pm, Rb, SyHa
WB, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Applications: WB, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0006.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0002.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0004.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] -Untreated (-) and treated (+) Neuro2A whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-12124_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-western-blot-41202413936..jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-28220241147219.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Staining of Hela cells with IRE1 alpha [p Ser724] Antibody (1F12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-282202412475125.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12123] - Untreated (-) and treated (+) HeLa whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12123-img0001.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12123] - Untreated (-) and treated (+) HeLa whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody diluted at 1:1000.")
![Western Blot-NBP3-22674-IRE1 alpha [p Ser724] Antibody (SR1062)-Analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22674_rabbit-ire1-alpha-p-ser-724-mab-sr1062-western-blot-158202371750..jpg "Western Blot-NBP3-22674-IRE1 alpha [p Ser724] Antibody (SR1062)-Analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.")
![Western Blot: IRE1 alpha [p Ser724] Antibody (S05-6E7) [NBP3-14946] - Western blot detection of IRE1 alpha [p Ser724] in MCF-7, HL-60 cell lysates using IRE1 alpha [p Ser724] Rabbit mAb (1:1000 diluted). Predicted band size: 110kDa. Observed band size: 110kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-p-Ser724-Antibody-S05-6E7-Western-Blot-NBP3-14946-img0001.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody (S05-6E7) [NBP3-14946] - Western blot detection of IRE1 alpha [p Ser724] in MCF-7, HL-60 cell lysates using IRE1 alpha [p Ser724] Rabbit mAb (1:1000 diluted). Predicted band size: 110kDa. Observed band size: 110kDa.")
![Western Blot: IRE1 alpha [p Ser724] Antibody [HRP] [NB100-2323H] - Detection of phosphorylated IRE-1 alpha using NB100-2323. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE1 alpha.](http://images.novusbio.com/fullsize2/IRE1-alpha-[p-Ser724]-Antibody-[HRP]-Western-Blot-NB100-2323H-img0006.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody [HRP] [NB100-2323H] - Detection of phosphorylated IRE-1 alpha using NB100-2323. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE1 alpha.")