Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S5p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Chromatin-Immunoprecipitation-NBP2-59219-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Cross reactivity of the antibody directed against Pol IIS5p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS5p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S5 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-ELISA-NBP2-59219-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Immunofluorescence-NBP2-59219-img0005.jpg)
![Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-CTD-4H8-Western-Blot-NBP2-50274-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.")
![Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-CTD-8A7-Western-Blot-NBP2-50243-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.")
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours or treated by nocodazole (50 ng/mL) at 37C for 20 hours or treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-10C7U2-Western-Blot-NBP3-16910-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours or treated by nocodazole (50 ng/mL) at 37C for 20 hours or treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")