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![Western Blot: cGAS [p Ser420] Antibody [NBP3-16951] - Western blot analysis of extracts of various cell lines, using cGAS antibody (NBP3-16951) at 1:1000 dilution.293T and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/cGAS-[p-Ser420]-Antibody-Western-Blot-NBP3-16951-img0001.jpg "Western Blot: cGAS [p Ser420] Antibody [NBP3-16951] - Western blot analysis of extracts of various cell lines, using cGAS antibody (NBP3-16951) at 1:1000 dilution.293T and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")