Product Search for novus: Novus Biologicals

Novus Biologicals products are now on bio-techne.com

Sort By:

Products (3)

page of 1

3 Results for "novus"

MAB1094

MSK1/MSK2 [p Ser376, p Ser360] Antibody (1013D) [Unconjugated]

(1 Publication)

100 μg

MAB1094

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Monoclonal Antibody (Catalog # MAB1094) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1 (S376)/MSK2 (S360) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nm PMA for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for MSK1/MSK2 at approximately 98 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Monoclonal Antibody (Catalog # MAB1094) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Time course of phosphorylation changes after clofarabine treatment of STA-ET-7.2 cells.(A) STA-ET-7.2 cells treated with 0.5μM clofarabine and total cell lysates collected at different time points. Western blot results showed increased phosphorylation starting as early as 3 minutes; (B) phosphorylation changes were sustained through 25 hours with a reduction around one hour. Actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34133426), licensed under a CC-BY license. Not internally tested by R&D Systems.

Phosphorylation changes after treatment for different ES and OS cell lines.(A) Cytarabine’s effect on STA-ET.7.2 cells compared to the clofarabine treatment. Cells were treated with clofarabine, cytarabine or DMSO. Immunoblot analysis showed cytarabine did not alter phosphorylation of ERK1/2, MSK1/2 and CREB; (B) Immunoblot results of phosphorylation changes after clofarabine treatment of different ES cell lines; clofarabine caused increased phosphorylation of ERK1/2, MSK1/2 and CREB in all five ES cell lines; (C) Clofarabine treated OS cell lines did not show significant increase in phosphorylation of ERK1/2, MSK1/2 and CREB. Actin was used as the loading control for all experiments. (CLF: Clofarabine, CYT: Cytarabine). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34133426), licensed under a CC-BY license. Not internally tested by R&D Systems.

Clofarabine and CD99 antibodies cause specific protein phosphorylation in ES cell lines.(A) STA-ET-7.2 cells treated with clofarabine and western blot analyses were done by phosphospecific antibodies. Phosphorylation of ERK1/2, MSK1/2 and CREB levels were increased with clofarabine treatment; (B) TC-71 cells treated with two different C99 antibodies (15μg/ml) and phosphorylation changes were analyzed by immunoblotting. Similar to clofarabine treatment, phosphorylation of ERK1/2, MSK1/2 and CREB were increased; (C, D) STA-ET-7.2 cells treated with clofarabine, CD 99 antibody and decreased phosphorylation changes for STAT3 and AMPK alpha 1 were validated by western blotting; (E) A4573 cells treated with clofarabine show increased level of pMSK1/2 and main phosphorylated protein is pMSK2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34133426), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1094-SP

compare

(select up to 3 products from this page to compare)

AF1094

MSK1/MSK2 [p Ser376, p Ser360] Antibody

(2 Publications)

100 μg

AF1094

Western blot shows lysates of HEK293 human embryonic kidney cell line untreated(-) or treated (+) with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) in the presence or absence of pathway inhibitors SB203580 and PD98059 for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1/MSK2 (MSK1 S376, MSK2 S360) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1094-SP

compare

(select up to 3 products from this page to compare)

MAB25181

MSK1/MSK2 [p Ser376, p Ser360] Antibody (1013F) [Unconjugated]

100 μg

MAB25181

MSK1/2phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated (top panel), or unstimulated (bottom panel), with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human MSK1/2 Monoclonal Antibody (Catalog # MAB25181) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MSK1/2phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with PMA using Rabbit Anti-Human MSK1/MSK2 Monoclonal Antibody (Catalog # MAB25181) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB25181-SP

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

©2024 Novus Biologicals, All Rights Reserved.