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![Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (NBP3-13099) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Paxillin-[p-Ser273]-Antibody-Western-Blot-NBP3-13099-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (NBP3-13099) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (NBP3-13099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Paxillin-[p-Ser273]-Antibody-Western-Blot-NBP3-13099-img0001.jpg)
![ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159] - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-[p-Ser273]-Antibody-ELISA-NBP2-44159-img0004.jpg "ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159] - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate.")