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![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-PhosphoRNA Polymerase II (S2) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0029.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0021.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Western-Blot-NB100-1805-img0022.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0023.jpg)
![Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Human ovarian carcinoma. Antibody: Affinity purified rabbit antiPhospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-Immunohistochemistry-Paraffin-NB100-1805-img0028.jpg)
![RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 used for IP at 5 ug per reaction and another Affinity purified rabbit anti-RNA Polymerase II antibody used for IP at 5 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, NB100-1805 was used at 0.1 ug/ml (upper panel) and the other antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser2]-Antibody-NB100-1805-img0030.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S2p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Chromatin-Immunoprecipitation-NBP2-59215-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Western-Blot-NBP2-59215-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - Cross reactivity of the antibody directed against Pol IIS2p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS2p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S2 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-ELISA-NBP2-59215-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser2] Antibody (C.15200005) [NBP2-59215] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser2-Antibody-C-15200005-Immunofluorescence-NBP2-59215-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] - Immunocytochemical labeling of CORO1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal CORO1B (C-terminus) and CORO1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23242_rabbit-coro1b-p-ser2-pab-11920231633163.jpg "Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] - Immunocytochemical labeling of CORO1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal CORO1B (C-terminus) and CORO1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.")
![Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] - Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-CORO1B (C-terminal region) (lanes 1 & 2) or anti-CORO1B (Ser-2) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23242_rabbit-coro1b-p-ser2-pab-189202317355215.jpg)