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![Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) [NBP2-61501] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61501 at 0.5 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-p-Ser14-Antibody-RM238-Western-Blot-NBP2-61501-img0004.jpg "Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) [NBP2-61501] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61501 at 0.5 ug/ml")
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) [NBP2-61501] - Immunocytochemistry of HeLa cells using NBP2-61501 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-p-Ser14-Antibody-RM238-Immunocytochemistry-Immunofluorescence-NBP2-61501-img0001.jpg)
![Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-Western-Blot-NBP2-62794-img0004.jpg "Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.")
![Dot Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To test the cross reactivity of the antibody against H2BS14p, an analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. This figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-Dot-Blot-NBP2-62794-img0003.jpg)
![ELISA: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To determine the titer of the antibody, analysis was performed using a serial dilution of the antibody against H2BS14p.The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-ELISA-NBP2-62794-img0002.jpg)