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![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: Synuclein-alpha [p Ser129] Antibody [NB300-746] - Rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/Synuclein-alpha-[p-Ser129]-Antibody-Western-Blot-NB300-746-img0001.jpg "Western Blot: Synuclein-alpha [p Ser129] Antibody [NB300-746] - Rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CREB-[p-Ser129]-Antibody-Western-Blot-NBP3-05463-img0001.jpg "Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of C6 cells, using CREB antibody (NBP3-05463) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CREB [p Ser129] Antibody-Western Blot-NBP3-05463-img0002.jpg)
![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP3-05429-img0001.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21567_rabbit-akt1-p-ser-129-mab-sr2047-5720231754841.jpg "Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.")
![Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/AKT1-p-Ser129-Antibody-7M4I8-Western-Blot-NBP3-16908-img0001.jpg "Western Blot: AKT1 [p Ser129] Antibody (7M4I8) [NBP3-16908] - Western blot analysis of extracts of MCF7 cells, using AKT1 Rabbit mAb (NBP3-16908) at 1:1000 dilution.MCF7 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.](http://images.novusbio.com/fullsize/nbp3-21526_rabbit-histone-h2a-p-ser-129-mab-sr1183-57202316112156.jpg "Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.")