| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1081AF405 | |||
| Alexa Fluor 488 | NB21-1081AF488 | |||
| Alexa Fluor 647 | NB21-1081AF647 | |||
| Alexa Fluor 700 | NB21-1081AF700 | |||
| Biotin | NB21-1081B | |||
| DyLight 405 | NB21-1081V | |||
| DyLight 488 | NB21-1081G | |||
| DyLight 550 | NB21-1081R | |||
| DyLight 650 | NB21-1081C | |||
| FITC | NB21-1081F | |||
| HRP | NB21-1081H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1081SS | |||
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NB21-1081-img0008.jpg "Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3K9ac/K14ac in C. elegans embryo lysate. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Histone H3.2 K9,K14ac antibody was tested at 1:50 in HeLa cells with FITC. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunocytochemistry-NB21-1081-img0012.jpg)
![Flow Cytometry: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Analysis of Alexa Fluor (R) 700 conjugate of NB21-1081. Jurkat cells stained with Alexa Fluor 700 conjugated acetylated Histone H3 antibody (blue) or isotype control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Flow-Cytometry-NB21-1081-img0013.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3 K9ac/K14ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NB21-1081-img0009.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NBP2-54617-img0003.jpg "Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - HeLa cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9/14ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunofluorescence-NBP2-54617-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - ChIP assays were performed using human HeLa cells, the antibody against H3K9/14ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-ac-Lys14-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54617-img0001.jpg)
![ELISA: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9/14ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-ELISA-NBP2-54617-img0002.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To test the cross reactivity of the antibody against H3K9/14ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NBP2-54617-img0006.jpg)
![Protein Array: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Protein-Array-NBP2-54617-img0005.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[ac-Lys9-ac-Lys14]-Kit-Chromatin-Immunoprecipitation-NBP1-71714-img0008.jpg "Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[ac-Lys9-ac-Lys14]-Kit-Chromatin-Immunoprecipitation-NBP1-71714-img0006.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[ac-Lys9-ac-Lys14]-Kit-Chromatin-Immunoprecipitation-NBP1-71714-img0007.jpg)