| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1074AF405 | |||
| Alexa Fluor 488 | NB21-1074AF488 | |||
| Alexa Fluor 647 | NB21-1074AF647 | |||
| Alexa Fluor 700 | NB21-1074AF700 | |||
| Biotin | NB21-1074B | |||
| DyLight 405 | NB21-1074V | |||
| DyLight 488 | NB21-1074G | |||
| DyLight 550 | NB21-1074R | |||
| DyLight 650 | NB21-1074C | |||
| FITC | NB21-1074F | |||
| HRP | NB21-1074H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1074SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-12179-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25994 | |||
![Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Dot-Blot-NB21-1074-img0011.jpg "Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1074-img0014.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of H3K9ac in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1074-img0018.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in HeLa histone prep. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0016.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0012.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-AH3-120-Western-Blot-NB120-12179-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa")
![Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Western-Blot-NBP2-61536-img0012.jpg "Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61536 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Immunocytochemistry-Immunofluorescence-NBP2-61536-img0010.jpg)
![Immunohistochemistry: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Immunohistochemistry staining of HepG2 cells using NBP2-61536.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Immunohistochemistry-NBP2-61536-img0011.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - ChIP performed on HeLa cells using 5 ug NBP2-61536. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Chromatin-Immunoprecipitation-NBP2-61536-img0014.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and NBP2-61536 at 1 ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-ELISA-NBP2-61536-img0007.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - NBP2-61536 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Multiplex-Immunoassay-NBP2-61536-img0013.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NBP2-59180-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NBP2-59180] - ChIP assays were performed using human HeLa cells, the antibody against H3K9ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers speci c for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that acetylation of K9 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59180-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180] - HeLa cells were stained with the antibody against H3K9ac and with DAPI. Cells were mixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immuno uorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunofluorescence-NBP2-59180-img0003.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody [NBP2-59180] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9ac in antigen coated wells. The antigen used was a peptide containing the histone modi cation of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,700.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-ELISA-NBP2-59180-img0005.jpg)
![Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Western-Blot-NBP2-43733-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [464] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Immunocytochemistry-Immunofluorescence-NBP2-43733-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunocytochemistry analysis of Histone H2AE antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/Histone-H2AE-ac-Lys9-Antibody-3H5-Immunocytochemistry-Immunofluorescence-NBP3-15226-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunocytochemistry analysis of Histone H2AE antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunocytochemistry/Immunofluorescence: Histone H2AE [ac Lys9] Antibody (3H5) [NBP3-15226] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2AE antibody 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Histone-H2AE-ac-Lys9-Antibody-3H5-Immunocytochemistry-Immunofluorescence-NBP3-15226-img0002.jpg)