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![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunofluorescence analysis of U2OS cells using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunocytochemistry-Immunofluorescence-NBP3-15933-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunofluorescence analysis of U2OS cells using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunohistochemistry-Paraffin-NBP3-15933-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunocytochemistry-Immunofluorescence-NBP3-15933-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunofluorescence analysis of PC-12 cells using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunocytochemistry-Immunofluorescence-NBP3-15933-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunohistochemistry of paraffin-embedded human breast cancer using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunohistochemistry-Paraffin-NBP3-15933-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (8V6Q6) [NBP3-15933] - Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit mAb (NBP3-15933) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-8V6Q6-Immunohistochemistry-Paraffin-NBP3-15933-img0002.jpg)
![Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-immunoprecipitation-622024101825..jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (2V10N10)- [NBP3-15933] -Analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-chromatin-immunoprecipitation-622024102021..jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (2V10N10)- [NBP3-15933] - Analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-chromatin-immunoprecipitation-622024102155..jpg)
![Dot Blot-Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933- Analysis of all sorts of peptides using Acetyl-Histone H3-K27 Rabbit mAb at 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-dot-blot-622024102348..jpg)
![Western Blot: -Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933-analysis of lysates from C2C12 cells using Acetyl-Histone H3-K27 Rabbit mAb at 1:100000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-western-blot-622024102550..jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Western-Blot-NBP2-54615-img0007.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-54615] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-54615-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human K562 cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0010.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive TSH2B and MYT1 genes, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0009.jpg)
![Dot Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Dot Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - To test the cross reactivity of the antibody against H3K27ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Dot-Blot-NBP2-54615-img0008.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-54615] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K27ac The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-54615-img0011.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Western-Blot-NBP2-59225-img0001.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-59225] - ChIP assays were performed using HeLa cells, the antibody against H3K27ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the active ACTB and EIF4A2 genes, used as positive controls, and for the coding region of the inactive MYT1 and TSH2B genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27 acetylation is enriched at the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59225-img0003.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-59225] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-59225-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-59225] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-59225-img0004.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Western-Blot-NBP3-13553-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Histone H3 (acetyl Lys27) antibody [GT261] detects Histone H3 (acetyl Lys27) protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Immunohistochemistry-Paraffin-NBP3-13553-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys27] Antibody (GT261) [NBP3-13553] - Histone H3 (acetyl Lys27) antibody [GT261] detects Histone H3 (acetyl Lys27) protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3 (acetyl Lys27) antibody [GT261] (NBP3-13553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-GT261-Immunohistochemistry-Paraffin-NBP3-13553-img0002.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Western-Blot-NBP2-61549-img0005.jpg "Western Blot: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61549 at 1 ug/ml, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa. Observed molecular weight is ~17 kDa")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61549 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Immunocytochemistry-Immunofluorescence-NBP2-61549-img0004.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys27] Antibody (RM172) [NBP2-61549] - NBP2-61549 specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-RM172-Multiplex-Immunoassay-NBP2-61549-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Immunofluorescence-NBP2-59302-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-Chromatin-Immunoprecipitation-NBP2-59302-img0002.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody (C.15200184) [NBP2-59302] - To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-C-15200184-ELISA-NBP2-59302-img0005.jpg)