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![Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Western-Blot-NBP3-15229-img0004.jpg "Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.")
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2B type 1B antibody 1:84, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Immunocytochemistry-Immunofluorescence-NBP3-15229-img0003.jpg)
![Flow Cytometry: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Overlay histogram showing Hela cells stained with Histone H2B type 1B antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Flow-Cytometry-NBP3-15229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunocytochemistry analysis of Histone H2B type 1B antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Immunocytochemistry-Immunofluorescence-NBP3-15229-img0002.jpg)
![Western Blot: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61559, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys20-Antibody-RM205-Western-Blot-NBP2-61559-img0010.jpg "Western Blot: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using NBP2-61559, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61559, red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys20-Antibody-RM205-Immunocytochemistry-Immunofluorescence-NBP2-61559-img0008.jpg)
![Multiplex Immunoassay: Histone H4 [ac Lys20] Antibody (RM205) [NBP2-61559] - NBP2-61559 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys20-Antibody-RM205-Multiplex-Immunoassay-NBP2-61559-img0011.jpg)
![Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-RM235-Western-Blot-NBP2-61496-img0004.jpg "Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml")
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61496 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-RM235-Immunocytochemistry-Immunofluorescence-NBP2-61496-img0001.jpg)
![Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - NBP2-61496 specifically reacts to Histone H2B type 1B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-RM235-Multiplex-Immunoassay-NBP2-61496-img0005.jpg)
![Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Western-Blot-NBP3-13538-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2B [ac Lys20] Antibody (GT578) [NBP3-13538] - Histone H2B (acetyl Lys20) antibody [GT578] detects Histone H2B (acetyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2B (acetyl Lys20) stained by Histone H2B (acetyl Lys20) antibody [GT578] (NBP3-13538) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Histone-H2B-ac-Lys20-Antibody-GT578-Immunocytochemistry-Immunofluorescence-NBP3-13538-img0002.jpg)