| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1144AF405 | |||
| Alexa Fluor 488 | NB21-1144AF488 | |||
| Alexa Fluor 647 | NB21-1144AF647 | |||
| Alexa Fluor 700 | NB21-1144AF700 | |||
| Biotin | NB21-1144B | |||
| DyLight 405 | NB21-1144V | |||
| DyLight 488 | NB21-1144G | |||
| DyLight 550 | NB21-1144R | |||
| DyLight 650 | NB21-1144C | |||
| FITC | NB21-1144F | |||
| HRP | NB21-1144H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1144SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25995 | |||
![Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Dot-Blot-NB21-1144-img0005.jpg "Dot Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in the peptides listed above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody [NB21-1144] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1144 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NB21-1144-img0010.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody [NB21-1144] - Analysis of Histone H3 K18ac in (1) HeLa histone prep, (2) NIH 3T3 histone prep and (3) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Western-Blot-NB21-1144-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys18] Antibody [NB21-1144] - Histone H3 [ac Lys18] antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1144-img0009.jpg)
![Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Western-Blot-NBP2-43535-img0010.jpg "Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43535-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse intestine. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0005.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![Dot Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27ac .](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Dot-Blot-NBP2-43535-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Western-Blot-NBP2-61538-img0011.jpg "Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61538 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Immunocytochemistry-Immunofluorescence-NBP2-61538-img0009.jpg)
![Immunohistochemistry: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Immunohistochemistry staining of HepG2 cells using NBP2-61538.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Immunohistochemistry-NBP2-61538-img0010.jpg)
![Chromatin Immunoprecipitation (ChIP): Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using 5 ug NBP2-61538. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Chromatin-Immunoprecipitation-ChIP-NBP2-61538-img0013.jpg)
![ELISA: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and biotinylated anti-H3K18ac NBP2-61538 at 2ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-ELISA-NBP2-61538-img0004.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - NBP2-61538 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Multiplex-Immunoassay-NBP2-61538-img0012.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3R17me2(asym)K18ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Western-Blot-NBP2-59218-img0001.jpg "Western Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3R17me2(asym)K18ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody [NBP2-59218] - ChIP assays were performed using human HeLa cells, the antibody against H3R17me2(asym)K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-59218-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - To test the cross reactivity of the antibody against H3R17me2(asym)K18ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3R17K18. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Dot-Blot-NBP2-59218-img0002.jpg)
![ELISA: Histone H3 [ac Lys18] Antibody [NBP2-59218] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3R17me2(asym) K18ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:28,800.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-ELISA-NBP2-59218-img0003.jpg)
![Immunofluorescence: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Mouse NIH3T3 cells were stained with the antibody against H3R17me2(asym)K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3R17me2(asym)K18ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Immunofluorescence-NBP2-59218-img0005.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)