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![Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Western-Blot-NBP2-59266-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NBP2-59266] - HeLa cells were stained with the antibody against H4K12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K12ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Immunofluorescence-NBP2-59266-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody [NBP2-59266] - ChIP assays were performed using human HeLa cells, the antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with the ChIP-seq kit on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59266-img0003.jpg)
![ELISA: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,300.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-ELISA-NBP2-59266-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To test the cross reactivity of the antibody against H4K12ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows the antibody is specific for the K12 acetylation with some slight cross reaction with K8ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Dot-Blot-NBP2-59266-img0007.jpg)
![Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Western-Blot-NBP2-59271-img0001.jpg "Western Blot: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - ChIP assays were performed using human HeLa cells, the antibody against H2BK12ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Chromatin-Immunoprecipitation-NBP2-59271-img0003.jpg)
![ELISA: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:38,200.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-ELISA-NBP2-59271-img0002.jpg)
![Immunofluorescence: Histone H2B [ac Lys12] Antibody [NBP2-59271] - Histone H2B Antibody [NBP2-59271] - HeLa cells were stained with the antibody against H2BK12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK12ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-Immunofluorescence-NBP2-59271-img0004.jpg)
![Western Blot: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61554, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-RM202-Western-Blot-NBP2-61554-img0006.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61554, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61554 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-RM202-Immunocytochemistry-Immunofluorescence-NBP2-61554-img0005.jpg)
![Multiplex Immunoassay: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - NBP2-61554 specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 12 (K126 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-RM202-Multiplex-Immunoassay-NBP2-61554-img0007.jpg)
![Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Western-Blot-NBP2-59241-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Chromatin-Immunoprecipitation-NBP2-59241-img0002.jpg)