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![Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Western-Blot-NBP2-59227-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - Western blot was performed on histone extracts (30 ug) from HeLa cells using the antibody against H3K64me3 . The antibody was diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - ChIP assays were performed using human K562 cells, the antibody against H3K64me3 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and Sata satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Chromatin-Immunoprecipitation-NBP2-59227-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K64me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:5,500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-ELISA-NBP2-59227-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys64] Antibody [NBP2-59227] - To check the specificity of the antibody against H3K64me3 a Dot Blot was performed with peptides containing different modifications of histone H3 and H4 or the unmodified H3K64 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys64]-Antibody-Dot-Blot-NBP2-59227-img0005.jpg)