| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1071AF405 | |||
| Alexa Fluor 488 | NB21-1071AF488 | |||
| Alexa Fluor 647 | NB21-1071AF647 | |||
| Alexa Fluor 700 | NB21-1071AF700 | |||
| Biotin | NB21-1071B | |||
| DyLight 405 | NB21-1071V | |||
| DyLight 488 | NB21-1071G | |||
| DyLight 550 | NB21-1071R | |||
| DyLight 650 | NB21-1071C | |||
| FITC | NB21-1071F | |||
| HRP | NB21-1071H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1071SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26001 | |||
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1071-img0009.jpg "Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1071 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1071-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Antibody at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1071-img0016.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - WB analysis of H3K9me1 in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0013.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit [NBP1-71713] - 2 ug of NB21-1023 was used to IP DNA from fixed HeLa cells along side a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Monomethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71713-img0007.jpg "Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit [NBP1-71713] - 2 ug of NB21-1023 was used to IP DNA from fixed HeLa cells along side a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit [NBP1-71713] - Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Monomethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71713-img0005.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Monomethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71713-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Western-Blot-NBP2-61535-img0007.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Immunocytochemistry of HeLa cells, using monomethyl-Histone H3 (Lys9) Rabbit mAb NBP2-61535 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Immunocytochemistry-Immunofluorescence-NBP2-61535-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - ChIP performed on HeLa cells using H3K9me1 antibody (NBP2-61535). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Chromatin-Immunoprecipitation-NBP2-61535-img0009.jpg)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - NBP2-61535 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Multiplex-Immunoassay-NBP2-61535-img0008.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NBP2-59204-img0001.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - ChIP assays were performed using human HeLa cells, the antibody directed against H3K9me1 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. Respectively 1 and 5 ug of the antibody and 5 ug of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NBP2-59204-img0004.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me1 with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Dot-Blot-NBP2-59204-img0002.jpg)
![ELISA: Histone H3 [Monomethyl Lys9] Antibody [NBP2-59204] - Histone H3 Antibody [NBP2-59204] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me1, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:68,000.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-ELISA-NBP2-59204-img0003.jpg)
![Other Histone H3 [Monomethyl Lys9] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Peptide-In-vitro-assay-NB21-1071PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys9] Antibody Blocking Peptide")
![Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-262202414471558.jpg "Immunohistochemistry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Image of HIST1H3A [Monomethyl Lys9] Antibody (13E1) diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Staining of A549 with HIST1H3A [Monomethyl Lys9] Antibody (13E1) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 489-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202412493612.jpg)
![Flow Cytometry: HIST1H3A [Monomethyl Lys9] Antibody (13E1) [NBP3-26135] - Overlay Peak curve showing A549 cells stained with HIST1H3A [Monomethyl Lys9] Antibody (13E1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26135_rabbit-hist1h3a-monomethyl-lys-9-mab-13e1-282202414183677.jpg)