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![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Western-Blot-NBP2-42859-img0003.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42859-img0002.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of anti-Histone H4 (mono methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (mono methyl Lys20) antibody 1:3000 dilution. A: Peptide samples of H4K20 un B: Peptide samples of H4K20me1 C: Peptide samples of H4K20me2 D: Peptide samples of H4K20me3.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Dot-Blot-NBP2-42859-img0001.jpg)