| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1072AF405 | |||
| Alexa Fluor 488 | NB21-1072AF488 | |||
| Alexa Fluor 647 | NB21-1072AF647 | |||
| Alexa Fluor 700 | NB21-1072AF700 | |||
| Biotin | NB21-1072B | |||
| DyLight 405 | NB21-1072V | |||
| DyLight 488 | NB21-1072G | |||
| DyLight 550 | NB21-1072R | |||
| DyLight 650 | NB21-1072C | |||
| FITC | NB21-1072F | |||
| HRP | NB21-1072H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1072SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25998 | |||
![Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Dot-Blot-NB21-1072-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1072-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of Histone H3K9me2 on HeLa histone preps. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1072-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Analysis of H3K9me2 in C. elegans embryo lysate. Observed molecular weight is ~13 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NB21-1072-img0012.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Dimethyl Lys9] ChIP Kit [NBP1-71712] - Chromata ChIP Histone H3 [Dimethyl Lys9] Kit [NBP1-71712] - 2 ug of NB21-1023 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Dimethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71712-img0008.jpg "Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Dimethyl Lys9] ChIP Kit [NBP1-71712] - Chromata ChIP Histone H3 [Dimethyl Lys9] Kit [NBP1-71712] - 2 ug of NB21-1023 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Dimethyl Lys9] ChIP Kit [NBP1-71712] - Chromata ChIP Histone H3 [Dimethyl Lys9] Kit [NBP1-71712] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa cell chromatin.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Dimethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71712-img0005.jpg)
![Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Dimethyl Lys9] Kit [NBP1-71712] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/Chromata-ChIP-Histone-H3-[Dimethyl-Lys9]-Kit-Chromatin-Immunoprecipitation-NBP1-71712-img0006.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NBP2-59200-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Mouse NIH3T3 cells were stained with the antibody against H3K9me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunofluorescence-NBP2-59200-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me2 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59200-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9me2 , crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-ELISA-NBP2-59200-img0002.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Western-Blot-NBP2-61534-img0007.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Immunocytochemistry of HeLa cells, using NBP2-61534 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Immunocytochemistry-Immunofluorescence-NBP2-61534-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - ChIP performed on HeLa cells using 5ug NBP2-61534. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Chromatin-Immunoprecipitation-NBP2-61534-img0009.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - NBP2-61534 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Multiplex-Immunoassay-NBP2-61534-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Western-Blot-NBP2-59294-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Immunofluorescence-NBP2-59294-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K9me3 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 3 and 9 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Chromatin-Immunoprecipitation-NBP2-59294-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Cross reactivity of the antibody directed against H3K9me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K9me2. The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-ELISA-NBP2-59294-img0002.jpg)
![Western Blot: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] - Positive Western Blot detected in Mouse liver tissue. All lanes: Histone H3.1 [Dimethyl Lys9] Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26621_rabbit-histone-h3-1-dimethyl-lys-9-mab-1a10-282202411485811.jpg "Western Blot: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] - Positive Western Blot detected in Mouse liver tissue. All lanes: Histone H3.1 [Dimethyl Lys9] Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys9] Antibody (1A10) [NBP3-26621] - Staining of Hela cells with Histone H3.1 [Dimethyl Lys9] Antibody (1A10) at 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26621_rabbit-histone-h3-1-dimethyl-lys-9-mab-1a10-28220241323638.jpg)