| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1022AF405 | |||
| Alexa Fluor 488 | NB21-1022AF488 | |||
| Alexa Fluor 647 | NB21-1022AF647 | |||
| Alexa Fluor 700 | NB21-1022AF700 | |||
| Biotin | NB21-1022B | |||
| DyLight 405 | NB21-1022V | |||
| DyLight 488 | NB21-1022G | |||
| DyLight 550 | NB21-1022R | |||
| DyLight 650 | NB21-1022C | |||
| FITC | NB21-1022F | |||
| HRP | NB21-1022H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1022SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25985 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1022AF405 | |||
| Alexa Fluor 488 | NB21-1022AF488 | |||
| Alexa Fluor 647 | NB21-1022AF647 | |||
| Biotin | NB21-1022B | |||
| DyLight 405 | NB21-1022V | |||
| DyLight 488 | NB21-1022G | |||
| DyLight 550 | NB21-1022R | |||
| DyLight 650 | NB21-1022C | |||
| FITC | NB21-1022F | |||
| HRP | NB21-1022H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1022 | |||
| BSA Free | NB21-1022SS | |||
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NB21-1022-img0001.jpg "Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1022 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1022-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Western blot analysis of H3 K4me2 in HeLa histone preps at 1:1000. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Histone H3 was tested at 1:100 in Hela cells against Dylight 488 (Green). Nuclei were illuminated blue with DAPI.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1022-img0007.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - H3K4Me2 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - WB analysis of H3 K4me2 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0004.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NBP2-59187-img0003.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Panel B, C, D and E: staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/uL blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K4me2 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. A: cells were immunofluorescently labeled with the H3K4me2 antibody (left) diluted 1:5,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - ChIP was performed with the antibody against H3K4me2 on sheared chromatin from 500,000 HeLaS3 cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration of the antibody of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0006.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K4me2 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:48,200.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-ELISA-NBP2-59187-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me2 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59187-img0002.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-RM135-Western-Blot-NBP2-61540-img0004.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61540 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-RM135-Immunocytochemistry-Immunofluorescence-NBP2-61540-img0003.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - NBP2-61540 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-RM135-Multiplex-Immunoassay-NBP2-61540-img0005.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Western-Blot-NBP2-59288-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Immunofluorescence-NBP2-59288-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me2 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Chromatin-Immunoprecipitation-NBP2-59288-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Cross reactivity of the antibody directed against H3K4me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me2. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the dimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-ELISA-NBP2-59288-img0002.jpg)
![Western Blot: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Positive Western Blot detected in Mouse brain tissue, Mouse heart tissue. All lanes: Histone H3.1 [Dimethyl Lys4] Antibody at 0.55ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26622_rabbit-histone-h3-1-dimethyl-lys-4-mab-6f6-28220241136305.jpg "Western Blot: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Positive Western Blot detected in Mouse brain tissue, Mouse heart tissue. All lanes: Histone H3.1 [Dimethyl Lys4] Antibody at 0.55ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Analysis of Histone H3.1 [Dimethyl Lys4] Antibody (6F6) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26622_rabbit-histone-h3-1-dimethyl-lys-4-mab-6f6-2822024132365.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Staining of Hela cells with Histone H3.1 [Dimethyl Lys4] Antibody (6F6) at 1:34, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26622_rabbit-histone-h3-1-dimethyl-lys-4-mab-6f6-28220241312245.jpg)
![Flow Cytometry: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Overlay histogram showing Hela cells stained with Histone H3.1 [Dimethyl Lys4] Antibody (6F6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26622_rabbit-histone-h3-1-dimethyl-lys-4-mab-6f6-282202414312754.jpg)
![Other Histone H3 [Dimethyl Lys4] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Peptide-Chromatin-Immunoprecipitation-NB21-1022PEP-img0002.jpg "Other Histone H3 [Dimethyl Lys4] Antibody Blocking Peptide")