| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [282] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Immunocytochemistry-Immunofluorescence-NBP2-42860-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of HIST1H4A protein from HeLa whole cell extracts using 5 ug of Histone H4K20me2 (dimethyl Lys20) antibody [282]. Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [282]. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Immunoprecipitation-NBP2-42860-img0004.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Dot blot analysis of various of Histone H4 [Dimethyl Lys20] Antibody (282) at antibody dilution of 1:500.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Dot-Blot-NBP2-42860-img0005.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Western-Blot-NBP2-42861-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [1851] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [1851] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Immunocytochemistry-Immunofluorescence-NBP2-42861-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Chromatin-Immunoprecipitation-NBP2-42861-img0001.jpg)
![Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Analysis of HIST1H4A protein from HeLa whole cell extracts using 5 ug of Histone H4K20me2 (dimethyl Lys20) antibody [1851]. Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Immunoprecipitation-NBP2-42861-img0004.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody (1851) [NBP2-42861] - Dot blot analysis of H4K20 methylation peptide using Histone H4 [Dimethyl Lys20] Antibody (1851) at antibody dilution of 1:500.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-1851-Dot-Blot-NBP2-42861-img0006.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Western-Blot-NBP2-59246-img0001.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K20me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - HeLa cells were stained with the antibody against H4K20me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4K20me2 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Immunofluorescence-NBP2-59246-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K20me2 and optimized PCR primer sets for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit on the IP-Star automated system, using sheared chromatin from 1 million cells. Respectively 1 and 5 ug of antibody was used per ChIP experiment. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Chromatin-Immunoprecipitation-NBP2-59246-img0002.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody (C.15200220) [NBP2-59246] - To test the cross reactivity of the antibody against H4K20me2, analysis was performed with peptides containing other histone modifications and the unmodified H4K20. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-C-15200220-Dot-Blot-NBP2-59246-img0004.jpg)
![Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Peptide-Chromatin-Immunoprecipitation-NB21-2089PEP-img0002.jpg "Other Histone H4 [Dimethyl Lys20] Antibody Blocking Peptide")