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![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Chromatin-Immunoprecipitation-NBP2-44066-img0001.jpg "Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.")
![Western Blot: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 Ug per lane. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Western-Blot-NBP2-44066-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44066-img0002.jpg)
![Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg9] Antibody Antibody. Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg9] Antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.](http://images.novusbio.com/fullsize/nbp2-44066_rabbit-polyclonal-histone-h3-asym-dimethyl-arg9-antibody-245202310564613.jpg)