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NB100-355

RPE65 Antibody (401.8B11.3D9) - BSA Free

(7 Reviews)

(93 Publications)

0.2 ml

NB100-355

Western Blot: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Subcellular localization of BEST1 and surface Ca2+-dependent Cl- current in patient-derived iPSC-RPEs. Western blots show similar BEST1 expression levels in WT and patient-derived iPSC-RPEs. Each sample was from one cell lysis (BEST1 and beta-actin, RPE65 and CRALBP were on two gels, respectively). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29914), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Expression of eye-specific markers in the induced eye-like structures induced from lignin-added ES cells. (a) Higher-magnification image of the RPE like structure induced from ESCs after 12-day culture. (b) Immunostaining of eye- like structures. Eye- like structures induced from ESCs after 12-day culture were stained with antibodies against RPE65 (red) and nuclei were stained with DAPI solution (blue). Scale bar: a = 50 um, b,d = 200 um, c,e = 100 um. PLoS One. 2013 Jun 21;8(6):e66376. doi: 10.1371/journal.pone.0066376.

Immunohistochemistry-Paraffin: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Analysis of FFPE human glioblastoma tissue section using 1:500 dilution of RPE65 [401.8B11.3D9] antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems).

$Western Blot: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.$

Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.

Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).

Immunohistochemistry: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Zebrafish brain ventricular area labeled with mouse anti-RPE65 (1:100). This image was submitted through a verified customer review.

Simple Western: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - iPSC-RPE from all donors express RPE marker proteins. iPSC-RPE from the patient, the patients mom, the patients dad, and the three unrelated, unaffected controls all expressed RPE65. The negative control (iPSCs) had no detectable RPE65 protein expression. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29540715/) licensed under a CC-BY license.

Knockout Validated: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Immunoblotting showing the expression of RPE-specific proteins BEST1 (NB300-164), RPE65 (NB100-355), CRALBP, and the loading control beta-Actin in iPSC-RPE cells. Two gels/blots in the same panel were prepared from the same cell lysate of each PSC-RPE to detect BEST1 + beta-Actin, and RPE65 + CRALBP, respectively. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34061021/) licensed under a CC-BY license.

Attenuation of mTORC1/2 kinase ablates CPT-induced p53- and p53-dependent responses in ccRCC. (a) The 786-O and 786-O/VHL cells were treated with 400 nM pp242, 2 μM CPT, 10 μM nutlin-3a (N3) or vehicle control (DMSO) alone for 24 h, or preincubated with 400 nM pp242 for 1 h before addition of 2 μM CPT or 10 μM N3 for a further 24 h. Whole-cell lysates were assayed by western blot for HIF-2 alpha , mTOR, phosphorylated p53 (S15), mTOR (S2448) and p70S6K (T389) proteins. Actin was used as a loading control. Short and long exposure times are shown for P-S15-p53. (b) RCC4 and RCC4/VHL cells were treated with 400 nM pp242 or vehicle control (DMSO) alone or preincubated with 400 nM pp242 for 1 h before addition of either 1 μM or 2 μM CPT for 24 h. Whole-cell lysates were assayed by western blot for mTOR, p53, phosphorylated p53 (S15), mTOR (S2448) and p70S6K (T389) proteins. Actin was used as a loading control. (c) RCC4 and RCC4/VHL cells were preincubated with 400 nM pp242 for 1 h before addition of 2 μM CPT for 24 h and mRNA expression of ET-1 and PAI-1 were assessed by real-time quantitative PCR relative to GAPDH. Mean±S.E. of duplicate values of one representative experiment is shown. (d) RCC4 cells were pretreated with 400 nM pp242 for 1 h before addition of 2 μM CPT for 24 h. Conditioned media were harvested and assessed for secreted ET-1 protein levels by ELISA and normalized to total protein levels. Mean±S.E. of duplicate values of one representative experiment is shown Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24136229), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Depletion of TCAB1 reduced the cell proliferation in vitro. a. qPCR results. Using exogenous shTCAB1 lentivirus (4 different shRNA targets) depleted TCAB1 mRNA significantly. b. Protein level also significantly decreased after shTCAB1 lentivirus treatment. c. Depletion of TCAB1 by exogenous shRNA decreased cell proliferation in Cal-27, ACC2 and HSC-3 cells. d. FACS results. Depletion of TCAB1 might facilitate cancer cell arrest, and shTCAB1-treated cells are significantly less in S phase. Statistical analysis was determined by Student’s t test (**P < 0.01, ***P < 0.005). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25070141), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Cortisol inhibited selective autophagy towards SCG10 via activation of mTOR.a The cells were treated with cortisol (1 μM) for 3 h, and stained with rhod-2 (3 μM) for 1 h to detect mitochondrial Ca2+. After incubation, mitotracker green (MTG, 300 nM) was also stained to visualize mitochondria. The intensity of both rhod-2 (red) and MTG (green) was measured with Eclipse Ts2™ fluorescence microscopy. ** indicates p < 0.01 vs. control. Scale bars represent 100 μm (magnification, ×400). n = 5. b The cells were treated with RU 486 (1 μM) for 30 min before cortisol (1 μM) for 6 h, and then reacted with ATP luciferase reagent. The ATP levels were detected with luminometer. ** indicates p < 0.01 vs. control and ## indicates p < 0.01 vs. cortisol alone. n = 6. c Time responses (0–6 h) of cortisol (1 μM) in phosphorylation of AMPK at Thr172 and mTOR at Ser2448 were shown. ** indicates p < 0.01 vs. control. n = 4. d The cells were treated with xestospongin C (1 μM) for 2 h or ruthenium red (100 nM) for 30 min before cortisol (1 μM) for 6 h. p-mTOR (Ser2448), mTOR, and beta -actin were detected in western blotting results. * indicates p < 0.05 vs. control and # indicates p < 0.05 vs. cortisol alone. n = 4. e The cells were pretreated with rapamycin (100 nM) for 30 min before cortisol (1 μM) for 24 h. Atg5, p62, LC3, and beta -actin were detected with western blot. ** indicates p < 0.01 vs. control. #, ## indicates p < 0.05, p < 0.01 vs. cortisol, respectively. n = 5. f Time responses (0–24 h) of cortisol (1 μM) in stathmin-1 and SCG10 expressions were shown. *, ** indicates p < 0.05, p < 0.01 vs. control, respectively. n = 5. g The cells were pretreated with rapamycin (100 nM) for 30 min before cortisol (1 μM) for 24 h. SCG10 and beta -actin were detected with western blot. * indicates p < 0.05 vs. control and ## indicates p < 0.01 vs. cortisol. n = 4. h The cells were pretreated with rapamycin (100 nM) for 30 min before cortisol (1 μM) for 24 h. SCG10 was co-immunoprecipitated with an anti-ubiquitin antibody (the left side). Expression of ubiquitin, SCG10, and beta -actin in total cell lysates is shown in the right side. ** indicates p < 0.01 vs. control and ## indicates p < 0.01 vs. cortisol alone. n = 4. i The cells were pretreated with rapamycin (100 nM) for 30 min before cortisol (1 μM) for 24 h. Co-localization of LC3 (green) and SCG10 (red) was visualized with SRRF imaging system. DAPI was used for nuclear counterstaining (blue). ** indicates p < 0.01 vs. control and ## indicates p < 0.01 vs. cortisol alone. Scale bars represent 20 μm (magnification, ×1,000). n = 5. All blot and immunofluorescence images are representative. Quantative data are presented as a mean ± S.E.M. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30429451), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Xp

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-355AF350
Alexa Fluor 405	NB100-355AF405
Alexa Fluor 488	NB100-355AF488
Alexa Fluor 532	NB100-355AF532
Alexa Fluor 594	NB100-355AF594
Alexa Fluor 647	NB100-355AF647
Alexa Fluor 700	NB100-355AF700
Alexa Fluor 750	NB100-355AF750
Allophycocyanin	NB100-355APC
Biotin	NB100-355B
DyLight 350	NB100-355UV
DyLight 405	NB100-355V
DyLight 488	NB100-355G
DyLight 550	NB100-355R
DyLight 594	NB100-355DL594
DyLight 650	NB100-355C
DyLight 680	NB100-355FR
DyLight 755	NB100-355IR
FITC	NB100-355F
HRP	NB100-355H
Janelia Fluor 549	NB100-355JF549
Janelia Fluor 646	NB100-355JF646
PE	NB100-355PE
PerCP	NB100-355PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-355SS
Azide and BSA Free	NBP2-80931

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NB100-355AF405

RPE65 Antibody (401.8B11.3D9) [Alexa Fluor® 405]

0.2 ml

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Xp

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor 405

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-355AF350
Alexa Fluor 488	NB100-355AF488
Alexa Fluor 532	NB100-355AF532
Alexa Fluor 594	NB100-355AF594
Alexa Fluor 647	NB100-355AF647
Alexa Fluor 700	NB100-355AF700
Alexa Fluor 750	NB100-355AF750
Allophycocyanin	NB100-355APC
Biotin	NB100-355B
DyLight 350	NB100-355UV
DyLight 405	NB100-355V
DyLight 488	NB100-355G
DyLight 550	NB100-355R
DyLight 594	NB100-355DL594
DyLight 650	NB100-355C
DyLight 680	NB100-355FR
DyLight 755	NB100-355IR
FITC	NB100-355F
HRP	NB100-355H
Janelia Fluor 549	NB100-355JF549
Janelia Fluor 646	NB100-355JF646
PE	NB100-355PE
PerCP	NB100-355PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-355SS
Azide and BSA Free	NBP2-80931
BSA Free	NB100-355

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