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![Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-28751-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>GW182 Antibody [NBP1-28751] - Detection of human GW182 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibodyNBP1-28751 (lot NBP1-28751-2) used for IP at 6 ug/mg lysate. GW182 was also immunoprecipitated by a previous lot (lot NBP1-28751-1) of this antibody. For blotting immunoprecipitated GW182, NBP1-28751 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-NBP1-28751-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: GW182 Antibody [NBP1-28751] - Downregulation of eIF4E2 increases the protein levels of endogenous IMP1. HeLa cells were transfected with siRNAs indicated. At 48 h posttransfection, cells were lysed. A fraction of the lysate was subjected to SDS-PAGE followed by Western blotting. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s13238-017-0444-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GW182-Antibody-Knockdown-Validated-NBP1-28751-img0005.jpg)
![Western Blot: GW182 Antibody [NBP1-57134] - NMDAR-dependent increase in Ago2-GW182 interaction and Ago2 phosphorylation at S387 requires Akt activity. NMDAR-stimulated increase in Ago2-GW182 interaction is Akt-dependent. Cortical neuronal cultures were treated with Akti-1/2 (Akt inhibitor), chelerythrine (Chel, PKC inhibitor) or CT99021 (GSK-3beta inhibitor) 20 min before NMDA or vehicle application. Lysates were prepared 10 min after NMDA washout and immunoprecipitated with Ago2 antibodies or control IgG as shown. Proteins were detected by Western blotting. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embj.201797943) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-57134-img0003.jpg "Western Blot: GW182 Antibody [NBP1-57134] - NMDAR-dependent increase in Ago2-GW182 interaction and Ago2 phosphorylation at S387 requires Akt activity. NMDAR-stimulated increase in Ago2-GW182 interaction is Akt-dependent. Cortical neuronal cultures were treated with Akti-1/2 (Akt inhibitor), chelerythrine (Chel, PKC inhibitor) or CT99021 (GSK-3beta inhibitor) 20 min before NMDA or vehicle application. Lysates were prepared 10 min after NMDA washout and immunoprecipitated with Ago2 antibodies or control IgG as shown. Proteins were detected by Western blotting. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embj.201797943) licensed under a CC-BY license.")
![Western Blot: GW182 Antibody [NBP1-57134] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/GW182-Antibody-Western-Blot-NBP1-57134-img0002.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human prostate shows strong nuclear combined with weaker granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0005.jpg "Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human prostate shows strong nuclear combined with weaker granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human endometrium shows strong nuclear and moderate cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0003.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0004.jpg)
![Immunohistochemistry-Paraffin: GW182 Antibody [NBP1-88231] - Staining of human skeletal muscle shows very weak nuclear and cytoplasmic positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunohistochemistry-Paraffin-NBP1-88231-img0006.jpg)
![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0003.jpg "Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0002.jpg)