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![Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0006.jpg "Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1919-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0009.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Indirect staining of rat neuronal/glial cell culture.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0007.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0010.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0009.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0016.jpg "Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ATP5A Antibody [NBP2-15512] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15512-img0013.jpg)
![Immunohistochemistry-Frozen: ATP5A Antibody [NBP2-15512] - Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Frozen-NBP2-15512-img0008.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0004.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0005.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0010.jpg)
![Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-15512-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Immunohistochemistry-Paraffin-NBP2-15512-img0007.jpg)
![Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NB100-2831-img0001.jpg "Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.")