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![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP2-98350-img0007.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Staining of mouse Carbonic Anhydrase VIII/CA8 in mouse brain with rabbit polyclonal antibody at 1:25000 dilution.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunohistochemistry-Paraffin-NBP2-98350-img0003.jpg)
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Mouse Carbonic Anhydrase VIII/CA8 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 0.5 ul anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunoprecipitation-NBP2-98350-img0005.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-86654] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP1-86654-img0006.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-86654] - Analysis in human cell line K562.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-86654] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunohistochemistry-Paraffin-NBP1-86654-img0001.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-86654] - Analysis in mouse cerebellum tissue.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP1-86654-img0007.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:38 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-008-Western-Blot-NBP2-89517-img0001.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:38 kDa")
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (008) [NBP2-89517] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-CA8 rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-008-Immunoprecipitation-NBP2-89517-img0002.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Western-Blot-NBP2-90710-img0003.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa")
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Immunoprecipitation-NBP2-90710-img0002.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-99319] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP2-99319-img0004.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-99319] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 38 kDa")
![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-99319] - Carbonic Anhydrase VIII/CA8 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Immunoprecipitation-NBP2-99319-img0003.jpg)
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-15657] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP2-15657-img0002.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-15657] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-54947] - Titration: 5.0ug/ml Positive Control: SK-MEL-2 cell lysate.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VIII-CA8-Antibody-Western-Blot-NBP1-54947-img0003.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP1-54947] - Titration: 5.0ug/ml Positive Control: SK-MEL-2 cell lysate.")