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![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Immunocytochemistry-Immunofluorescence-NBP2-54609-img0022.jpg "Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Surface Plasmon Resonance: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - A synthesized biotin-labeled 5-mC conjugate was immobilized on a sensorchip. Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control, while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 uL/min. Interaction assays involved injections of 2 different dilutions of the 5-mC monoclonal antibody over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 minute washing step with HBS-N buffer. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH 3). The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Ovalbumine-Surface-Plasmon-Resonance-NBP2-54609-img0015.jpg)
![Dot Blot: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - To demonstrate the specificity of the antibody against 5-mC, a Dot blot analysis was performed using hmC, mC and C controls. 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. The antibody was used at a dilution of 1:300. Figure shows a high specificity of the antibody for the methylated control.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Dot-Blot-NBP2-54609-img0018.jpg)
![Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (33D3) [NBP2-54609] - Analysis was performed on 1 ug fragmented human genomic DNA using 0.2 ug of the monoclonal antibody against 5-mC. The fragmented DNA was spiked with controls (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with primer sets specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. The figure shows the recovery expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-33D3-Methylated-DNA-Immunoprecipitation-NBP2-54609-img0023.jpg)
![ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-ELISA-NBP2-90985-img0002.jpg "ELISA: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.")
![HPLC: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.NBP2-90985) was greater than 95% as determined by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-HPLC-NBP2-90985-img0003.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Recombinant SARS-COV-2 Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-SDS-Page-NBP2-90985-img0005.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 His (C-Term) Protein [NBP2-90985] - Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-His-C-Term-Protein-Surface-Plasmon-Resonance-NBP2-90985-img0004.jpg)
![Western Blot: Jagged 1 Antibody (J1G53-3) [NBP3-11671] -](http://images.novusbio.com/fullsize/Jagged-1-Antibody-J1G53-3-Western-Blot-NBP3-11671-img0003.jpg "Western Blot: Jagged 1 Antibody (J1G53-3) [NBP3-11671] -")
![Immunohistochemistry: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - Immunohistochemical staining of JAG1 using Jagged 1 antibody (J1G53-3) in Pancreas, Acini and Duct (2.5 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-J1G53-3-Immunohistochemistry-NBP3-11671-img0001.jpg)
![Flow Cytometry: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - FACS staining of human Lymphocyte(CD4+) using Jagged 1 antibody (J1G53-3) (PerCP).](http://images.novusbio.com/fullsize/Jagged-1-Antibody-J1G53-3-Flow-Cytometry-NBP3-11671-img0002.jpg)
![Surface Plasmon Resonance: Jagged 1 Antibody (J1G53-3) [NBP3-11671] - SPR analysis of the interaction between Jagged 1 antibody (J1G53-3) derivative hJagged1-Fc fusion protein. SPR response was taken when 13, 6.5, 3.25, 1.625, and 0.8125nM of J1G53-3 were injected onto the hJagged1-Fc fusion protein. J1G53-3 exhibited the highest binding affinity, and its property and binding ability was studied in detail by spectroscopy and surface Plasmon resonance (SPR). The result acquired through SPR was as follows: Kd value was 1.08nM and value of chi-square was 0.0237 through SPR analysis.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-J1G53-3-Surface-Plasmon-Resonance-NBP3-11671-img0004.jpg)
![ELISA: Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein [NBP3-07930] - Immobilized Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein, His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-RBD-N501Y-Protein-ELISA-NBP3-07930-img0001.jpg "ELISA: Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein [NBP3-07930] - Immobilized Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein, His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.")
![HPLC: Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein [NBP3-07930] - The purity of Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein is greater than 95% as determiend by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-RBD-N501Y-Protein-HPLC-NBP3-07930-img0002.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein [NBP3-07930] - Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein on Tris-Bis PAGE under reduced condition. The purity is great than 95%.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-RBD-N501Y-Protein-SDS-Page-NBP3-07930-img0004.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein [NBP3-07930] - Human ACE2 captured in protein A chip, can bind Recombinant SARS-CoV-2 Spike RBD (N501Y) Protein, His Tag with an affinity constant of 1.74nM as determined in SPR assap.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-RBD-N501Y-Protein-Surface-Plasmon-Resonance-NBP3-07930-img0003.jpg)
![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)
![SDS-Page: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-SDS-Page-NBP2-90982-img0001.jpg "SDS-Page: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.")
![ELISA: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-ELISA-NBP2-90982-img0002.jpg)
![HPLC: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - The purity of SARS-COV-2 Spike RBD Protein with His tag (Cat.NBP2-90982) was greater than 95% as determined by SEC-HPLC.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-HPLC-NBP2-90982-img0003.jpg)
![Surface Plasmon Resonance: Recombinant SARS-CoV-2 Spike S1 (RBD) His (C-Term) Protein [NBP2-90982] - Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-Spike-S1-RBD-His-C-Term-Protein-Surface-Plasmon-Resonance-NBP2-90982-img0004.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-ELISA-NBP3-05764-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Surface Plasmon Resonance: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - Cat. No. NBP3-05764, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-Surface-Plasmon-Resonance-NBP3-05764-img0003.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-ELISA-NBP3-05764-img0002.jpg)