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AF8125

Apolipoprotein A-IV/ApoA4 Antibody [Unconjugated]

100 μg

AF8125

Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human ileum (left panel) and descending colon (right panel) using Sheep Anti-Human/Mouse Apolipoprotein A‑IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).

Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF8125-SP

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NBP2-52930

Apolipoprotein A-IV/ApoA4 Antibody (CL0465)

0.1 ml

NBP2-52930

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Lane 1: Marker [kDa], Lane 2: Human plasma

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human colon shows strong positivity in plasma.

Immunohistochemistry: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human kidney shows strong immunoreactivity in renal tubules.

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (CL0465) [NBP2-52930] - Staining of human pancreas shows absence of immunoreactivity.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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