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![Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.](http://images.novusbio.com/fullsize/Malondialdehyde-Antibody-11E3-Western-Blot-NBP2-59367-img0009.jpg "Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.")
![Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Malondialdehyde-Antibody-11E3-Immunocytochemistry-Immunofluorescence-NBP2-59367-img0011.jpg)
![Immunohistochemistry: Malondialdehyde Antibody (11E3) [NBP2-59367] - Immunohistochemistry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 10X.](http://images.novusbio.com/fullsize/Malondialdehyde-Antibody-11E3-Immunohistochemistry-NBP2-59367-img0010.jpg)
![Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Malondialdehyde-Antibody-11E3-Western-Blot-NBP2-59367-img0008.jpg)