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NBP1-06713

OLLAS Epitope Tag Antibody (L2) - BSA Free

(2 Reviews)

(42 Publications)

0.1 ml

NBP1-06713

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - The A08a neuron receives arbor-specific synaptic inputs. Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. Image using the DyLight 650 form of this antibody (NBP1-06713C).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG (TM) and ANTI-FLAG (TM) are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - OLLAS tagged SFO-p41 protein.

Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. IHC image submitted by a verified customer review.

Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS

Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha-MES-4 antibody and K76 alpha-PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30201), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top), nSyb::CD19 (middle) and CD19::Sdc (bottom), with the R17H02 driver (in green). The brain samples were co-immunostained with antibodies against the OLLAS tag (present in ligand) & against the pre-synaptic protein, BRP (in magenta). The ligand proteins are co-localized with or adjacent to BRP (arrows), demonstrating that all ligands are enriched at the presynaptic terminals of the ORNs; CD19::Nrx was also expressed at strong levels in the axon shaft outside of the glomerulus (arrowheads in top panels). Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/32027), licensed under a CC-BY license.

Localization of P granule proteins during zygote polarization.(A) Photomicrographs of live wild-type (mCherry::MEX-5 and GLH-1::eGFP) or fixed meg-4 zygotes (MEG::3 OLLAS and PGL-1) at three different stages: before polarization (pronuclear formation), during polarization (pronuclear migration) and after polarization (mitosis). meg-4(ax3052) zygotes were co-immunostained for MEG-3::OLLAS (anti-OLLAS, Novus Biological), and PGL-1 (K76, DSHB). meg-4 is required redundantly with meg-3 for P granule assembly, and each is sufficient to support localized granule assembly (Wang et al., 2014). In this and subsequent figures, dashed lines outline each embryo, embryos are oriented with anterior to the left and posterior to the right and are ~50 uM long. At least three embryos were examined per genotype shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27914198), licensed under a CC-BY licence.

MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. C) Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha-MES-4 antibody and K76 alpha-PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29111977), licensed under a CC-BY licence.

Utilization of pMVP for the creation of unique PDX1 vectors. (B) Immunoblot blot analysis of INS1 832/13 cell lysates harvested 48h after treatment with crude adenovirus lysates. For the epitope tagged conditions, note the appearance of the endogenous (lower) and overexpressed (upper) PDX1 bands (top blot). For eGFP blot, the use of P2A adds 23 amino acids to the N-terminal protein (i.e. eGFP). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30590691), licensed under a CC-BY licence.

Reactivity:

ET

Species Glossary

Reactivity: ET

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-06713AF350
Alexa Fluor 405	NBP1-06713AF405
Alexa Fluor 488	NBP1-06713AF488
Alexa Fluor 532	NBP1-06713AF532
Alexa Fluor 594	NBP1-06713AF594
Alexa Fluor 647	NBP1-06713AF647
Alexa Fluor 700	NBP1-06713AF700
Alexa Fluor 750	NBP1-06713AF750
Biotin	NBP1-06713B
DyLight 350	NBP1-06713UV
DyLight 405	NBP1-06713V
DyLight 488	NBP1-06713G
DyLight 550	NBP1-06713R
DyLight 594	NBP1-06713DL594
DyLight 650	NBP1-06713C
DyLight 680	NBP1-06713FR
DyLight 755	NBP1-06713IR
FITC	NBP1-06713F
HRP	NBP1-06713H
Janelia Fluor 549	NBP1-06713JF549
Janelia Fluor 646	NBP1-06713JF646
mFluor Violet 450 SE	NBP1-06713MFV450
mFluor Violet 500 SE	NBP1-06713MFV500
mFluor Violet 610 SE	NBP1-06713MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-06713SS
Azide and BSA Free	NBP2-80893

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NBP2-16840

Histone H3 [Trimethyl Lys27] Antibody

(1 Review)

(2 Publications)

0.1 ml

NBP2-16840

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Observed molecular weight is ~15 kDa.

Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control).

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at a dilution of 1:1000. Observed molecular weight is ~15kDa.

Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Sample (30 ug of whole cell lysate) A: Jurkat, B: Raji, C: K562, D: THP-1, 7.5% SDS PAGE diluted at 1:500. Observed molecular weight is ~19 kDa.

Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A (tri-Methyl K27) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Immunofluorescence photomicrographs of frozen sections of mouse liver. Red: Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody Blue: Nuclear staining with Hoechst 33342.

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Hela xenograft, using Histon H3 (tri-Methyl K27) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse brain. Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse duodenum. Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry Whole-Mount: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3K27me3 (trimethyl Lys27) antibody dilution: 1:100.

Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at 1:2500.

Reactivity:

Hu, Mu, Rt, Ze, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ze, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-16865

hnRNP AB Antibody

0.1 ml

NBP2-16865

Western Blot: hnRNP AB Antibody [NBP2-16865] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE w diluted at 1:1000

Immunocytochemistry/Immunofluorescence: hnRNP AB Antibody [NBP2-16865] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A/B protein stained by hnRNP A/B antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP2-16865] - A549 xenograft, using HNRNPAB antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Knockout Validated: hnRNP AB Antibody [NBP2-16865] - Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Whole-Mount : hnRNP AB Antibody [NBP2-16865] -Fixed zebrafish embryos. hnRNP AB antibody dilution: 1:200.

Reactivity:

Hu, Ze, Mu, Rt, Po, Bv, Ch, RM

Species Glossary

Reactivity: Hu, Ze, Mu, Rt, Po, Bv, Ch, RM

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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