Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Knockdown Validated: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Knockdown-Validated-NB400-115-img0007.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: ABCF2 Antibody [NB400-115] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and ABCF2 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human ABCF2 Polyclonal Antibody (Catalog # NB400-115) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for ABCF2 at approximately 71 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NB400-115-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-115-img0005.jpg)
![Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Simple-Western-NB400-115-img0006.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89317] - Staining in human testis and liver tissues using anti-ABCF2 antibody. Corresponding ABCF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89317-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89317] - Staining in human testis and liver tissues using anti-ABCF2 antibody. Corresponding ABCF2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: ABCF2 Antibody [NBP1-89317] - Analysis using Anti-ABCF2 antibody NBP1-89317 (A) shows similar pattern to independent antibody NBP1-89316 (B).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89317-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NBP1-89317] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89317-img0006.jpg)
![Western Blot: ABCF2 Antibody [NBP1-89317] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89317-img0005.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89317] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89317-img0008.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89317] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89317-img0009.jpg)
![Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP2-98977-img0003.jpg "Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa")
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NBP2-98977] - Immunofluorescence staining of ABCF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ABCF2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98977-img0002.jpg)
![Immunoprecipitation: ABCF2 Antibody [NBP2-98977] - ABCF2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 1 ul anti-ABCF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ABCF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunoprecipitation-NBP2-98977-img0004.jpg)