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![In vitro binding assays of [V14]6 and [AP1-V12]6 polymer.(A, B) H226, (C, D) MDA-MB-231 and (E, F) H460 cells were incubated with 10 µM of [V14]6, [AP1-V12]6 and AP1 for 1 h at 4°C. Cell binding was determined using flow cytometry. Histograms are representative of three independent experiments. Graphical bars (on right) represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, **P<0.001, and *P<0.05, one-way ANOVA; n = 3. (G, H) H226 cells (3×105 cells) were pre- incubated with different concentrations (1, 5 and 10 µg/mL) of anti-IL-4 receptor antibody followed by 1 h incubation with 10 µM Alexa-labeled [AP1-V12]6 at 4°C. The cells were further suspended in 300 µL of PBS after washing and analyzed using flow cytometry. Histograms are representative of three independent experiments. Graphical bars represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, One way ANOVA; n = 3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab230_human-il-4-r-alpha-mab-clone-25463-4120241048249.jpg)
![In vivo, ex vivo imaging and biodistribution of [AP1-V12]6 polymers.(A) Cy5.5-labeled [V14]6 and [AP1-V12]6 (8 mg/kg) were intravenously injected into MDA-MB-231 tumor xenografted nude mice. Biodistribution was determined by collecting in vivo fluorescence images at different time points. Scale bar indicates normalized fluorescence intensity (NC). Representative optical images of three experiments. (B) Quantitation of fluorescence intensities in tumor sites at respective time points (n = 3). (C) Analysis of fluorescence intensities for tumors and organs from ex vivo images (n = 3). (D) Histological analysis of [AP1-V12]6 polymer (red) localization in tumors. Nuclei were stained with DAPI (blue), and IL-4R expression on cells was visualized by anti-IL-4 receptor antibody staining (green). Representative confocal images of three experiments (scale bar 50 µm). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab230_human-il-4-r-alpha-mab-clone-25463-41202410485426.jpg)
