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![Flow Cytometry: HypoxiTRAK(TM) [NBP3-18531] - Flow cytometry of -metabolite, blue & red excitation; intact A549 cells under hypoxia (3%, 1%), with & without [4 day co-exposure]](http://images.novusbio.com/fullsize/HypoxiTRAK-TM-Flow-Cytometry-NBP3-18531-img0001.jpg "Flow Cytometry: HypoxiTRAK(TM) [NBP3-18531] - Flow cytometry of -metabolite, blue & red excitation; intact A549 cells under hypoxia (3%, 1%), with & without [4 day co-exposure]")
![Immunocytochemistry/Immunofluorescence: HypoxiTRAK(TM) [NBP3-18531] - -metabolite accumulates in cytoplasm of A549 cells (ex. 633 nm; em. 680/20 nm) exposed to 100 nM for 4 days 1% O2](http://images.novusbio.com/fullsize/HypoxiTRAK-TM-Immunocytochemistry-Immunofluorescence-NBP3-18531-img0003.jpg)
![Flow Cytometry: HypoxiTRAK(TM) [NBP3-18531] - -metabolite persistence: intact A549 cells retain >70% after washing and 24h incubation in -free medium (ex. 633nm; em. >695nm)](http://images.novusbio.com/fullsize/HypoxiTRAK-TM-Flow-Cytometry-NBP3-18531-img0002.jpg)
![HypoxiTRAK(TM) [NBP3-18531] - Intracellular accumulation of HypoxiTRAK(TM)-metabolite is sensitive to degree of hypoxia and [HypoxiTRAK] (A549 cells, conditions as image 2)](http://images.novusbio.com/fullsize/HypoxiTRAK-TM-N-A-NBP3-18531-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HypoxiTRAK(TM) [NBP3-18531] - Multi-Cellular Tumor Spheroid (MCTS) generated from 5000 cells (Cal33, HNC) in a ULA well. MCTS grown for 3 days then probed with 50 nM HypoxiTRAK(TM) for a further 3 days. False-color heatmap image shows hypoxia reported in a cortical pattern and with a hypocellular centre (by Hoechst staining consistent with controls, not shown).Courtesy of DA Close & PA Johnston - University of Pittsburgh, School of Pharmacy.](http://images.novusbio.com/fullsize/HypoxiTRAK-TM-Immunocytochemistry-Immunofluorescence-NBP3-18531-img0005.jpg)