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![Western Blot: Human IgE Antibody (Fc-2001) [NBP1-78601] - Analysis of 0.5ug of purified human IgE, IgM, IgA and IgG per well and 10ul of PageRuler Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/Human-IgE-Antibody-Fc-2001-Western-Blot-NBP1-78601-img0004.jpg "Western Blot: Human IgE Antibody (Fc-2001) [NBP1-78601] - Analysis of 0.5ug of purified human IgE, IgM, IgA and IgG per well and 10ul of PageRuler Prestained Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - Vero cells were seeded on coverslips and infected with Yellow Fever 17D virus for 24 h at MOI 2. Control coverslips consisted of uninfected cells. After fixation with 4% PFA, samples were stained with the antibodies. Antibody was used at a dilution of 1:1000 dilution and Triton X-100 was used as detergent. Imaging was performed using a Leica SP5 confocal microscope (Image by Virology Research Services Ltd).](http://images.novusbio.com/fullsize/Yellow-Fever-Virus-NS1-Antibody-CE6-A-B9-G3-Immunocytochemistry-Immunofluorescence-NBP3-14846-img0001.jpg "Immunocytochemistry/Immunofluorescence: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - Vero cells were seeded on coverslips and infected with Yellow Fever 17D virus for 24 h at MOI 2. Control coverslips consisted of uninfected cells. After fixation with 4% PFA, samples were stained with the antibodies. Antibody was used at a dilution of 1:1000 dilution and Triton X-100 was used as detergent. Imaging was performed using a Leica SP5 confocal microscope (Image by Virology Research Services Ltd).")
![Direct ELISA: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - Assay showing specificity of Yellow Fever Virus NS1 antibody for Yellow Fever Virus NS1. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected at 0.01ug/ml, visualized with Goat anti-mouse IgG:HRP.](http://images.novusbio.com/fullsize/Yellow-Fever-Virus-NS1-Antibody-CE6-A-B9-G3-Direct-ELISA-NBP3-14846-img0002.jpg)
![Sandwich ELISA: Yellow Fever Virus NS1 Antibody (CE6.A.B9.G3) [NBP3-14846] - ELISA plates coated with Mouse Anti-Yellow Fever Virus NS1 antibody were used to capture YFV NS1 protein followed by detection with biotinylated Yellow Fever Virus NS1 antibody.](http://images.novusbio.com/fullsize/Yellow-Fever-Virus-NS1-Antibody-CE6-A-B9-G3-Sandwich-ELISA-NBP3-14846-img0003.jpg)
![SDS-Page: Cytomegalovirus Native Protein [NBP3-14808] - Coomassie-stained SDS-PAGE showing Cytomegalovirus purified antigen.](http://images.novusbio.com/fullsize/Cytomegalovirus-Native-Protein-SDS-Page-NBP3-14808-img0001.jpg "SDS-Page: Cytomegalovirus Native Protein [NBP3-14808] - Coomassie-stained SDS-PAGE showing Cytomegalovirus purified antigen.")
![Direct ELISA: Cytomegalovirus Native Protein [NBP3-14808] - Plate was coated with 1000ng, 100ng, 10ng or 1ng of NRP-2 and probed with 500ug/well of NRP-2, gH pentamer or gB huFc-tag and then blocked for 1 h at RT. Plate was probed with 500ug/well of NRP-2, gH pentamer or gB huFc-tag for 2h at RT. For primary antibodies, sheep anti-gH pentamer serum was used at 1:2,000, mouse anti-His-tag antibody was used at 1:1,200 (for ZIKV-NS1), no primary antibody was used for gB. Secondary antibody was used for gH pentamer at 1:2,000; gB huFc-tag detection via Fc-tag at 1:2,000 and ZIKV-NS1 at 1:2,000. All secondary incubations were done for 45 min at RT. Detection was carried out using TMB Membrane substrate (KPL) for 40 min. 1M HCl was used to stop the reaction. Assay confimred that NRP-2 binds specifically to gH pentamer but not to gB or ZIKV NS1.](http://images.novusbio.com/fullsize/Cytomegalovirus-Native-Protein-Direct-ELISA-NBP3-14808-img0002.jpg)
![Direct ELISA: Dengue Virus 3 NS1 Antibody (CC6.BC5.D8) [NBP3-14845] - Assay showing specificity of Dengue Virus 3 NS1 antibody for Dengue Fever Virus Serotype 3 NS1. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected at 0.01ug/ml, visualized with Goat anti-mouse IgG:HRP.](http://images.novusbio.com/fullsize/Dengue-Virus-3-NS1-Antibody-CC6-BC5-D8-Direct-ELISA-NBP3-14845-img0001.jpg "Direct ELISA: Dengue Virus 3 NS1 Antibody (CC6.BC5.D8) [NBP3-14845] - Assay showing specificity of Dengue Virus 3 NS1 antibody for Dengue Fever Virus Serotype 3 NS1. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected at 0.01ug/ml, visualized with Goat anti-mouse IgG:HRP.")
![Sandwich ELISA: Dengue Virus 3 NS1 Antibody (CC6.BC5.D8) [NBP3-14845] - Assay using Mouse Anti-Dengue Virus Pan-Serotype NS1 antibody as a capture antibody for DENV3 NS1 and biotin-labelled Dengue Virus 3 NS1 antibody as the detection antibody.](http://images.novusbio.com/fullsize/Dengue-Virus-3-NS1-Antibody-CC6-BC5-D8-Sandwich-ELISA-NBP3-14845-img0002.jpg)