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![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0015.jpg "Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Cytometry-NB100-662-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0009.jpg)
![Immunocytochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-NB100-662-img0005.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0006.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis of Alexa Fluor (R) 647 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1%](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0007.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0008.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on A549 cells with NB100-662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0011.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on SH-SY5Y cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0012.jpg)
![CyTOF-reported: rRNA Antibody (Y10b) [NB100-662] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-CyTOF-reported-NB100-662-img0014.jpg)
![Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of 50 ug mouse brain lysate/extract by 7.5% SDS-PAGE. O-GlcNAc transferase antibody [6G8] at 1:500.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-678-Western-Blot-NBP2-43590-img0002.jpg "Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of 50 ug mouse brain lysate/extract by 7.5% SDS-PAGE. O-GlcNAc transferase antibody [6G8] at 1:500.")
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [678] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-678-Immunocytochemistry-Immunofluorescence-NBP2-43590-img0003.jpg)
![Western Blot: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Analysis of 30 ug PC-12 whole cell lysate/extract by 7.5% SDS-PAGE. O-GlcNAc transferase antibody [6G8] at 1:500.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-678-Western-Blot-NBP2-43590-img0001.jpg)
![CyTOF-reported: O-GlcNAc Transferase p110 subunit Antibody (678) [NBP2-43590] - Mouse spleen cells were analyzed using NBP2-43590-167Er at 1:100. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-678-CyTOF-reported-NBP2-43590-img0004.jpg)
![Western Blot: O-GlcNAc Transferase p110 subunit Antibody (2037) [NBP2-43755] - Analysis of A. 50 ug rat brain whole cell lysate/extract 7.5 % SDS-PAGE O-GlcNAc transferase antibody [2037] dilution: 1:500](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-2037-Western-Blot-NBP2-43755-img0003.jpg "Western Blot: O-GlcNAc Transferase p110 subunit Antibody (2037) [NBP2-43755] - Analysis of A. 50 ug rat brain whole cell lysate/extract 7.5 % SDS-PAGE O-GlcNAc transferase antibody [2037] dilution: 1:500")
![Western Blot: O-GlcNAc Transferase p110 subunit Antibody (2037) [NBP2-43755] - Analysis of A. 30 ug 293T B. 30 ug HeLa 7.5 % SDS-PAGE O-GlcNAc transferase antibody [2037] dilution: 1:1000.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-2037-Western-Blot-NBP2-43755-img0001.jpg)
![Western Blot: O-GlcNAc Transferase p110 subunit Antibody (2037) [NBP2-43755] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE O-GlcNAc transferase antibody [2037] dilution: 1:1000](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-2037-Western-Blot-NBP2-43755-img0002.jpg)
![CyTOF-reported: O-GlcNAc Transferase p110 subunit Antibody (2037) [NBP2-43755] - Mouse spleen cells were analyzed using NBP2-43755-167Er at 1:100 and IL12-169Tm at 1:100. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-2037-CyTOF-reported-NBP2-43755-img0005.jpg)
![Flow Cytometry: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Analysis using the FITC conjugate of NBP1-28429. Staining of granulocytes.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-22A5-Flow-Cytometry-NBP1-28429-img0002.jpg "Flow Cytometry: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Analysis using the FITC conjugate of NBP1-28429. Staining of granulocytes.")
![CyTOF-reported: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Human bone marrow underwent RBC lysis and surface staining with Gd-160 conjugate of CD13 (22A5) antibody at 2 ug/mL, followed by gating on standard surface markers. Neutrophils demonstrate characteristic CD13 loss and reacquisition, with partial reactivity on monocytes. Specificity is very good, with minimal reactivity on lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-22A5-CyTOF-reported-NBP1-28429-img0004.jpg)