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![Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Western-Blot-NBP1-78619-img0003.jpg "Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.")
![Chemiluminescence Immunoassay: Digoxin Antibody (26G10) [NBP1-78619] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Chemiluminescence-Immunoassay-NBP1-78619-img0001.jpg)
![ELISA: Digoxin Antibody (26G10) [NBP1-78619] - Indirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-ELISA-NBP1-78619-img0002.jpg)
![Western Blot: Digoxin Antibody (38G12) [NBP1-78618] - Analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-Western-Blot-NBP1-78618-img0003.jpg "Western Blot: Digoxin Antibody (38G12) [NBP1-78618] - Analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.")
![Chemiluminescence Immunoassay: Digoxin Antibody (38G12) [NBP1-78618] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-Chemiluminescence-Immunoassay-NBP1-78618-img0001.jpg)
![ELISA: Digoxin Antibody (38G12) [NBP1-78618] - InDirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-ELISA-NBP1-78618-img0002.jpg)