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![Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/PF1-Antibody-Western-Blot-NB100-81671-img0003.jpg "Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81671 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81670. For blotting immunoprecipitated PF1, NB100-81671 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PF1-Antibody-Immunoprecipitation-NB100-81671-img0004.jpg)
![Immunoprecipitation: PF1 Antibody [NB100-81670] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81670 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81671. For blotting immunoprecipitated PF1, NB100-81670 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PF1-Antibody-NB100-81670-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: PF1 Antibody [NB100-81670] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81670 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81671. For blotting immunoprecipitated PF1, NB100-81670 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")