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![Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47256-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). Image courtesy of Dr. Matt Fuller at University of Missouri-Columbia.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47256-img0002.jpg)
![Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47257-img0002.jpg "Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody NBP1-47257 used for WB at 0.04 ug/mL (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47256, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/DNA-polymerase-alpha-Antibody-Western-Blot-NBP1-47257-img0001.jpg)