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8 Results for "novus"

NBP2-20710

TRIM25 Antibody

(1 Publication)

0.1 ml

NBP2-20710

Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP2-20710] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: TRIM25 protein stained by TRIM25 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Human colon carcinoma, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TRIM25 Antibody [NBP2-20710] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody diluted at 1:500.

Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: TRIM25 Antibody [NBP2-20710] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 ug of TRIM25 antibody 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Knockdown Validated: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.

Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. (a) E6-1 (Jurkat), dominant negative FADD (dnFADD) Jurkat, and overexpressing BCL-2 Jurkat were treated for 48 hours then stained for Annexin V and PI (b) E6-1 cells were plated and treated with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK for 48 hours. Cells were stained for Annexin V and PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V and PI (yellow), or negative for both Annexin V and PI (blue). Values are expressed as a mean ± SD from three independent experiments. (c) E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. (d) NHF and NCM460 cells were treated for 48 hours and 72 hours respectively, lysed and subjected to Western blot analysis. Bands were visualized with a chemiluminescence reagent. Images are representative of three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison. *p < 0.05 vs % viable of Control (DMSO); #p < 0.05 vs % viable cells for groups without Z-VAD(oMe)FMK. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28439094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effects of mBMSC and EA treatment on the expression of trophic factors. (A) Photomicrographs and (B‒D) histograms for mBDNF, NT4, and VEGF expression (n = 4–5). mBDNF expression was markedly increased by treatment with mBMSC and/or EA compared to vehicle treatment. mBDNF expression levels were higher in the EA-treated MCAO group than in the mBMSC-treated MCAO group. NT4 expression in the SVZ and hippocampus was significantly higher in the combined mBMSC and EA treatment group than in the other groups. VEGF expression in the hippocampus was markedly higher in the mBMSC treatment group than in the vehicle-treated MCAO group. #p < 0.05, ##p < 0.01, and ###p < 0.001 versus control group; *p < 0.05, **p < 0.01, and ***p < 0.001 versus vehicle-treated MCAO group; &p < 0.05, &&p < 0.01, and &&&p < 0.001 versus mBMSC-treated MCAO group; Scale bar = 1 mm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29391466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Mechanical tensile strain of 30% induces an elongation of paranodal junction and alteration in MAPK signaling in organotypic cerebellar slice culture. a Double immunostaining of organotypic cerebellar slices for CASPR in red and MAG in green. The response was evaluated at time 0 h post-stretch. Altered paranodal junctions are highlighted within white circles in high-magnification images. Scale bar, 50 μm; inset scale bar, 5 μm. b Sketch of CASPR and MAG labeling in the paranodal junction. Quantitative analysis is characterized by three length measurements: c nodal length called (A); d paranodal length called (B); and e (B-A) corresponding to the total length of the CASPR labeling. f–h Proteins were extracted following strain of 30% from control (Ctrl) and stretched (Stretch) slices and western blot was performed for the myelin protein MBP (f), ERK1/2 (g), and p38 (h). The ratio P-ERK on total ERK and the ratio P-p38 on total p38 are presented. beta -Actin was used as a loading control. Results represent the mean ± SEM (n = 3). ***p < 0.001. CASPR contactin-associated protein, MAG myelin-associated glycoprotein Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30298339), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines and detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 and A2; Right, Laemmli buffer, B1 and B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-84891

TRIM25 Antibody

0.1 ml

NBP1-84891

Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-84891] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-32050

TRIM25 Antibody

100 ul

NBP1-32050

Western Blot: TRIM25 Antibody [NBP1-32050] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal diluted at 1:500.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-32050] - HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: TRIM25 protein stained by TRIM25 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-32050] - Human breast cancer, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-21633

TRIM25 Antibody (SR2171)

100 ul

NBP3-21633

Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-76825

TRIM25 Antibody - BSA Free

0.1 mg

NBP1-76825

Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - HeLa cells with TRIM25 antibody at 20 ug/ml.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76825AF350
Alexa Fluor 405	NBP1-76825AF405
Alexa Fluor 488	NBP1-76825AF488
Alexa Fluor 532	NBP1-76825AF532
Alexa Fluor 594	NBP1-76825AF594
Alexa Fluor 647	NBP1-76825AF647
Alexa Fluor 700	NBP1-76825AF700
Alexa Fluor 750	NBP1-76825AF750
Biotin	NBP1-76825B
DyLight 350	NBP1-76825UV
DyLight 405	NBP1-76825V
DyLight 488	NBP1-76825G
DyLight 550	NBP1-76825R
DyLight 594	NBP1-76825DL594
DyLight 650	NBP1-76825C
DyLight 680	NBP1-76825FR
DyLight 755	NBP1-76825IR
FITC	NBP1-76825F
HRP	NBP1-76825H
Janelia Fluor 549	NBP1-76825JF549
Janelia Fluor 646	NBP1-76825JF646
PerCP	NBP1-76825PCP
mFluor Violet 450 SE	NBP1-76825MFV450
mFluor Violet 500 SE	NBP1-76825MFV500
mFluor Violet 610 SE	NBP1-76825MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76825-0.025mg

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NBP1-76826

TRIM25 Antibody - BSA Free

0.1 mg

NBP1-76826

Western Blot: TRIM25 Antibody [NBP1-76826] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76826] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76826] - HeLa cells with TRIM25 antibody at 5 ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76826AF350
Alexa Fluor 405	NBP1-76826AF405
Alexa Fluor 488	NBP1-76826AF488
Alexa Fluor 532	NBP1-76826AF532
Alexa Fluor 594	NBP1-76826AF594
Alexa Fluor 647	NBP1-76826AF647
Alexa Fluor 700	NBP1-76826AF700
Alexa Fluor 750	NBP1-76826AF750
Biotin	NBP1-76826B
DyLight 350	NBP1-76826UV
DyLight 405	NBP1-76826V
DyLight 488	NBP1-76826G
DyLight 550	NBP1-76826R
DyLight 594	NBP1-76826DL594
DyLight 650	NBP1-76826C
DyLight 680	NBP1-76826FR
DyLight 755	NBP1-76826IR
FITC	NBP1-76826F
HRP	NBP1-76826H
Janelia Fluor 549	NBP1-76826JF549
Janelia Fluor 646	NBP1-76826JF646
PerCP	NBP1-76826PCP
mFluor Violet 450 SE	NBP1-76826MFV450
mFluor Violet 500 SE	NBP1-76826MFV500
mFluor Violet 610 SE	NBP1-76826MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76826-0.025mg

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NBP1-00113

TRIM25 Antibody

0.1 ml

NBP1-00113

Western Blot: TRIM25 Antibody [NBP1-00113] - Detection of Human TRIM25 on HeLa whole cell lysate using NBP1-00113. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibodies NBP1-00114 and NBP1-00115.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-16382

TRIM25 Antibody (4Q0D2)

100 ul

NBP3-16382

Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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