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![Immunocytochemistry/Immunofluorescence: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25538_rabbit-itk-mab-hl1264-azide-and-bsa-free-212024940847.jpg "Immunocytochemistry/Immunofluorescence: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (NBP3-25538) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25538_rabbit-itk-mab-hl1264-azide-and-bsa-free-212024939150.jpg)
![Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25538_rabbit-itk-mab-hl1264-azide-and-bsa-free-212024940857.jpg)
![Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25538_rabbit-itk-mab-hl1264-azide-and-bsa-free-212024939178.jpg)
![Western Blot: ITK Antibody [NBP1-31732] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ITK-Antibody-Western-Blot-NBP1-31732-img0007.jpg "Western Blot: ITK Antibody [NBP1-31732] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ITK Antibody [NBP1-31732] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ITK stained by ITK antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ITK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31732-img0009.jpg)
![Western Blot: ITK Antibody (SR2174) [NBP3-21762] - Western blot analysis using NBP3-21762 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21762_rabbit-itk-mab-sr2174-5720231721513.jpg "Western Blot: ITK Antibody (SR2174) [NBP3-21762] - Western blot analysis using NBP3-21762 in Jurkat cell lysate.")
![Western Blot: ITK Antibody [NBP2-32240] - Samples: Whole cell lysate (50 ug) from HeLa (RIPA), 293T (RIPA), and Jurkat (RIPA) cells. Antibodies: Affinity purified rabbit anti-ITK antibody NBP2-32240 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ITK-Antibody-Western-Blot-NBP2-32240-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ITK Antibody [NBP2-32240] - Samples: Whole cell lysate (50 ug) from HeLa (RIPA), 293T (RIPA), and Jurkat (RIPA) cells. Antibodies: Affinity purified rabbit anti-ITK antibody NBP2-32240 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: ITK Antibody [NBP3-17873] - Staining of human cell line JURKAT shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/ITK-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17873-img0001.jpg "Immunocytochemistry/Immunofluorescence: ITK Antibody [NBP3-17873] - Staining of human cell line JURKAT shows localization to plasma membrane & cytosol.")
![Immunohistochemistry: ITK Antibody (8F10) [NBP3-15208] - IHC image of ITK antibody diluted at 1:100 and staining in paraffin-embedded human lymph node tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/ITK-Antibody-8F10-Immunohistochemistry-NBP3-15208-img0001.jpg "Immunohistochemistry: ITK Antibody (8F10) [NBP3-15208] - IHC image of ITK antibody diluted at 1:100 and staining in paraffin-embedded human lymph node tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")