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NBP1-47801

GRP75/HSPA9B/Mortalin Antibody (OTI9F8)

(1 Publication)

0.1 ml

NBP1-47801

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRP75/HSPA9B/Mortalin.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.

Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70963AF350
Alexa Fluor 405	NBP2-70963AF405
Alexa Fluor 488	NBP2-70963AF488
Alexa Fluor 532	NBP2-70963AF532
Alexa Fluor 594	NBP2-70963AF594
Alexa Fluor 647	NBP2-70963AF647
Alexa Fluor 700	NBP2-70963AF700
Alexa Fluor 750	NBP2-70963AF750
Allophycocyanin	NBP2-70963APC
Biotin	NBP2-70963B
DyLight 350	NBP2-70963UV
DyLight 405	NBP2-70963V
DyLight 488	NBP2-70963G
DyLight 550	NBP2-70963R
DyLight 594	NBP2-70963DL594
DyLight 650	NBP2-70963C
DyLight 680	NBP2-70963FR
DyLight 755	NBP2-70963IR
FITC	NBP2-70963F
HRP	NBP2-70963H
Janelia Fluor 549	NBP2-70963JF549
Janelia Fluor 646	NBP2-70963JF646
PE	NBP2-70963PE
PerCP	NBP2-70963PCP
mFluor Violet 450 SE	NBP2-70963MFV450
mFluor Violet 500 SE	NBP2-70963MFV500
mFluor Violet 610 SE	NBP2-70963MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70963

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NBP1-31596

GRP75/HSPA9B/Mortalin Antibody

0.1 ml

NBP1-31596

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Whole cell extract (6 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat brain. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Mouse duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 ug of GRP75 antibody. Western blot analysis was performed using GRP75 antibody diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Reactivity:

Hu, Mu, Rt, Po, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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