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![Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Western-Blot-NBP1-46829-img0002.jpg "Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRPM7 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TRPM7 was also immunoprecipitated by rabbit anti-TRPM7 antibody BL9785, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 minute (B).](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0001.jpg)
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Detection of human TRPM7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRPM7 antibody NBP1-46829 (lot NBP1-46829-3) used for IP at 6 ug per reaction. TRPM7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46829-2). For blotting immunoprecipitated TRPM7, NBP1-46829 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0003.jpg)
![Immunohistochemistry-Paraffin: TRPM7 Antibody [NB100-810] - (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunohistochemistry-Paraffin-NB100-810-img0004.jpg "Immunohistochemistry-Paraffin: TRPM7 Antibody [NB100-810] - (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.")
![Western Blot: TRPM7 Antibody (SR1322) [NBP3-21852] - Western blot analysis using NBP3-21852 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21852_rabbit-trpm7-mab-sr1322-57202315123411.jpg "Western Blot: TRPM7 Antibody (SR1322) [NBP3-21852] - Western blot analysis using NBP3-21852 in HeLa cell lysate.")
![Western Blot: TRPM7 [p Ser1569] Antibody [NBP3-23192] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23192_rabbit-trpm7-p-ser1569-pab-19920231856911.jpg "Western Blot: TRPM7 [p Ser1569] Antibody [NBP3-23192] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).")
![Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23186_rabbit-trpm7-p-ser1513-pab-199202312523634.jpg "Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).")
![Western Blot: TRPM7 Antibody (M573) [NBP3-23439] - Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.](http://images.novusbio.com/fullsize/nbp3-23439_mouse-trpm7-mab-m573-11920231633167.jpg "Western Blot: TRPM7 Antibody (M573) [NBP3-23439] - Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.")
![Western Blot: TRPM7 Antibody [NBP3-23361] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23361_rabbit-trpm7-pab-119202316331624.jpg "Western Blot: TRPM7 Antibody [NBP3-23361] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).")
![Western Blot: TRPM7 [p Ser1493] Antibody [NBP3-23179] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23179_rabbit-trpm7-p-ser1493-pab-11920231633161.jpg "Western Blot: TRPM7 [p Ser1493] Antibody [NBP3-23179] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).")
![Western Blot: TRPM7 Antibody [NBP3-23362] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23362_rabbit-trpm7-pab-19920231252368.jpg "Western Blot: TRPM7 Antibody [NBP3-23362] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).")