Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Pm,
SyHa,
Gp,
Pm-Or,
Rb,
Sh,
XpSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, Xp
WB, Simple Western, IHC, ICC/IF (-), IP (-)
Applications: WB, Simple Western, IHC, ICC/IF (-), IP (-)
Host: Rabbit Polyclonal
![Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Simple-Western-NB600-532-img0016.jpg "Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: beta-Actin Antibody [NB600-532] - Detection of actin in 3T3 lysates (20ug). ECL detection 30 seconds. A specific band is seen using different dilutions: Lane 1 (1:15,000), Lane 2 (1:10,000), and Lane 3 (1:15,000).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0009.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-532 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0014.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Mouse colon whole cell lysate. PVDF membrane was probed with Rabbit Anti-B actin Antibody (Catalog # NB600-532) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0019.jpg)
![Immunohistochemistry: beta-Actin Antibody [NB600-532] - Detection of human Cytoskeletal Actin by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin (NB600-532). Detection: DAB](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-NB600-532-img0015.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using RCC4 whole cell lysate [NBP1-30412].](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0006.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0007.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Analysis using the HRP conjugate of NB600-532. Detection of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0011.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Treatments of periplocin and/or TRAIL activate DR4, FADD, and proapoptotic proteins in HCC cells. The effect of periplocin treatment on the expression of DR4, DR5, and FADD was analyzed by western blot. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ecam/2013/958025/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0018.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Hypoxic preconditioning increases PBMNC sensitivity to apelin-13 via upregulation of APJ expression, leading to growth factor secretion.(a) APJ protein expression was significantly increased in hypoxic PBMNCs. *p Mol Neurodegener (2020). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-532_rabbit-polyclonal-beta-actin-antibody-23220248135226.jpg)
![Western Blot: beta-Actin Antibody (BLR057F) [NBP2-76367] - Detection of human and mouse Cytoskeletal Actin by Western Blot of U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-Cytoskeletal Actin recombinant monoclonal [BLR057F] (NBP2-76367). Secondary: HRP-conjugated goat anti-rabbit IgG..](http://images.novusbio.com/fullsize/beta-Actin-Antibody-BLR057F-Western-Blot-NBP2-76367-img0003.jpg "Western Blot: beta-Actin Antibody (BLR057F) [NBP2-76367] - Detection of human and mouse Cytoskeletal Actin by Western Blot of U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-Cytoskeletal Actin recombinant monoclonal [BLR057F] (NBP2-76367). Secondary: HRP-conjugated goat anti-rabbit IgG..")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (BLR057F) [NBP2-76367] - Detection of human Cytoskeletal Actin in FFPE HeLa cells by immunocytochemistry. Antibody: Rabbit anti-Cytoskeletal Actin recombinant monoclonal [BLR057F] (NBP2-76367) Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-BLR057F-Immunocytochemistry-NBP2-76367-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Actin Antibody (BLR057F) [NBP2-76367] - Detection of human Cytoskeletal Actin in FFPE ovarian carcinoma by immunohistochemistry. Antibody: Rabbit anti-Cytoskeletal Actin recombinant monoclonal [BLR057F] (NBP2-76367). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-BLR057F-Immunohistochemistry-NBP2-76367-img0001.jpg)
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-20720231348119.jpg%3Fv=1681426096 "Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).")
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-19920231410116.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-119202316331610.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).")
![Western Blot: beta-Actin Antibody [NBP3-23319] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-19920231915417.jpg)
![Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-199202312403723.jpg "Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-19920231911470.jpg)
![Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of various cell lines, using beta-Actin antibody (NBP3-16886) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-4C4-Western-Blot-NBP3-16886-img0001.jpg "Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of various cell lines, using beta-Actin antibody (NBP3-16886) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of HeLa cells, using beta-Actin antibody (NBP3-16886) at 1:10000-1:320000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-4C4-Western-Blot-NBP3-16886-img0002.jpg)
![Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]Western blot of A431 cells, using anti-beta -Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of beta -Actin at the predicted MW (42 kDa).](http://images.novusbio.com/fullsize/nbp3-18847_rabbit-beta-actin-mab-rm112-161120231301818.png "Western Blot: beta-Actin Antibody (RM112)[NBP3-18847]Western blot of A431 cells, using anti-beta -Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of beta -Actin at the predicted MW (42 kDa).")
![Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-19920231820297.jpg "Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] - Immunocytochemical labeling of beta-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-beta-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight (R) 594.](http://images.novusbio.com/fullsize/nbp3-23324_mouse-beta-actin-mab-m008-1992023141019.jpg)
![SDS-Page: Recombinant Human beta-Actin Protein [H00000060-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Actin-Protein-SDS-Page-H00000060-P01-img0002.jpg "SDS-Page: Recombinant Human beta-Actin Protein [H00000060-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Beta Actin Overexpression Lysate (Adult Normal) [NBP2-10939] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Beta Actin.](http://images.novusbio.com/fullsize/Beta-Actin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10939-img0001.jpg "Western Blot: Beta Actin Overexpression Lysate (Adult Normal) [NBP2-10939] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Beta Actin.")
