Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: UCH-L3 Antibody [NBP1-86037] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP1-86037-img0005.jpg "Western Blot: UCH-L3 Antibody [NBP1-86037] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: UCH-L3 Antibody [NBP1-86037] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86037-img0006.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-86037-img0011.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-86037-img0002.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-86037-img0007.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-86037-img0008.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-86037] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-86037-img0009.jpg)
![Western Blot: UCH-L3 Antibody [NBP1-49711] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody NBP1-49711 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody NBP1-49710, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP1-49711-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: UCH-L3 Antibody [NBP1-49711] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody NBP1-49711 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody NBP1-49710, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-49711] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- UCH-L3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-NBP1-49711-img0003.jpg)
![Western Blot: UCH-L3 Antibody [NBP2-98339] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP2-98339-img0005.jpg "Western Blot: UCH-L3 Antibody [NBP2-98339] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339] - Immunochemical staining of mouse UCH-L3 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP2-98339-img0004.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-98339] - Immunochemical staining of mouse UCH-L3 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP2-98339-img0003.jpg)
![Immunoprecipitation: UCH-L3 Antibody [NBP2-98339] - Mouse UCH-L3 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. Lane C: 0.5 mg Jurkat Whole Cell Lysate. Lane D: 0.5 mg SH-SY5Y Whole Cell Lysate 2 ul anti-Mouse UCH-L3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse UCH-L3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunoprecipitation-NBP2-98339-img0006.jpg)
![Western Blot: UCH-L3 Antibody [NBP1-55316] - MCF7 cell lysate, Antibody Titration: 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP1-55316-img0001.jpg "Western Blot: UCH-L3 Antibody [NBP1-55316] - MCF7 cell lysate, Antibody Titration: 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316] - Human placenta tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-55316-img0003.jpg)
![Western Blot: UCH-L3 Antibody [NBP1-55316] - Antibody Positive control: Lane 1 : 30ug Zebrafish skin lysate Lane 2: 30ug Zebrafish liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1 : 4000.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP1-55316-img0004.jpg)
![Western Blot: UCH-L3 Antibody (007) [NBP2-90878] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:31 kDa.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-007-Western-Blot-NBP2-90878-img0004.jpg "Western Blot: UCH-L3 Antibody (007) [NBP2-90878] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:31 kDa.")
![Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878] - Immunochemical staining of rat UCHL3 in rat testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-007-Immunohistochemistry-Paraffin-NBP2-90878-img0003.jpg)
![Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878] - rat UCHL3 was immunoprecipitated using: Lane A:0.5 mg k562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. 2 uL anti-rat UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-rat UCHL3 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-007-Immunoprecipitation-NBP2-90878-img0005.jpg)
![Western Blot: UCH-L3 Antibody (153) [NBP2-90679] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa. (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/UCH-L3-Antibody-153-Western-Blot-NBP2-90679-img0004.jpg "Western Blot: UCH-L3 Antibody (153) [NBP2-90679] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa. (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679] - Mouse UCHL3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg 293T Whole Cell Lysate. 2 uL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse UCHL3 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/UCH-L3-Antibody-153-Immunoprecipitation-NBP2-90679-img0003.jpg)
![Western Blot: UCH-L3 Antibody [NBP2-99525] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP2-99525-img0003.jpg "Western Blot: UCH-L3 Antibody [NBP2-99525] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa")
![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP2-99525] - Immunochemical staining of human UCH-L3 in human skeletal muscle with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP2-99525-img0002.jpg)
![Western Blot: UCH-L3 Antibody [NBP2-99847] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 14 kDa](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Western-Blot-NBP2-99847-img0002.jpg "Western Blot: UCH-L3 Antibody [NBP2-99847] - Anti-UCH-L3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 14 kDa")
![Immunoprecipitation: UCH-L3 Antibody [NBP2-99847] - UCH-L3 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-UCH-L3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UCH-L3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/UCH-L3-Antibody-Immunoprecipitation-NBP2-99847-img0001.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody (204) [NBP2-90680] - Immunochemical staining of mouse UCHL3 in mouse bone with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-204-Immunohistochemistry-Paraffin-NBP2-90680-img0003.jpg "Immunohistochemistry-Paraffin: UCH-L3 Antibody (204) [NBP2-90680] - Immunochemical staining of mouse UCHL3 in mouse bone with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: UCH-L3 Antibody (204) [NBP2-90680] - Immunochemical staining of mouse UCHL3 in mouse heart with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-204-Immunohistochemistry-Paraffin-NBP2-90680-img0001.jpg)
![Immunohistochemistry-Paraffin: UCH-L3 Antibody (204) [NBP2-90680] - Immunochemical staining of mouse UCHL3 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/UCH-L3-Antibody-204-Immunohistochemistry-Paraffin-NBP2-90680-img0002.jpg)