| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 5860-AP-010/CF | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | BAF884 | |||
| Phycoerythrin | IC884P | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC884P | |||
| Carrier Free | AF884-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5860-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8843-SP | |||
| Carrier Free | IC8843S-100UG | |||
| Carrier Free | IC8843V-100UG | |||
| Carrier Free | IC8843R-100UG | |||
| Carrier Free | IC8843U-100UG | |||
| Carrier Free | IC8843N-100UG | |||
| Carrier Free | IC8843T-100UG | |||
| Carrier Free | IC8843G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35145-1mg | |||
| Carrier-Free | NBP2-35145-100ug | |||
| Carrier-Free | NBP2-35145-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76767-0.025mg | |||
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Western-Blot-NBP1-97587-img0006.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Flow-Cytometry-NBP1-97587-img0004.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0002.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41389-018-0058-2) licensed under a CC-BY license.")
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Detection of endogenous human APRIL with MAb to APRIL (human) (Aprily-5). Method: Cell lysate (A) or cell supernatant (B) were run on an SDS-PAGE gel and analyzed with 2ug/ml Aprily-5 by Western blot. Dendritic cells (1), NIH3T3 (2) and NIH3T3 expressing human APRIL (3).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-5-Western-Blot-NBP1-97599-img0001.jpg)
![Breast cancer cells induce secretion of APRIL from neutrophils. Following approval by the University of Calgary's Conjoint Health Research Ethics Board, blood was obtained from healthy volunteers that gave informed consent. Neutrophils were isolated as described previously48,49 and suspended in Hank's Balanced Salt Solution with Ca2+/Mg2+ (Gibco). BCCs (Hs578T, SKBR3, T47D, and MCF7) obtained from the American Type Culture Collection (not authenticated but tested for mycoplasma in the current year) were cultured in Dulbecco's Modified Eagle Medium (Gibco) with 10% fetal bovine serum (Gibco) and 100 U/ml each of penicillin and streptomycin (Gibco) in 5% CO2 at 37 C. a APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody (ED, MyBioSource: MBS194153). On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29904116), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nbp1-97599_mouse-monoclonal-april-tnfsf13-antibody-aprily-5-291220231215618.jpg)
![Western Blot: APRIL/TNFSF13 Antibody [NBP1-76767] - Jurkat cells with TNFSF13 antibody at 1 ug/ml (A), 2 ug/ml (B), and 4 ug/ml (C).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Western-Blot-NBP1-76767-img0004.jpg "Western Blot: APRIL/TNFSF13 Antibody [NBP1-76767] - Jurkat cells with TNFSF13 antibody at 1 ug/ml (A), 2 ug/ml (B), and 4 ug/ml (C).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody [NBP1-76767] - Analysis of APRIL in human prostate tissue using NBP1-76767 at 10 ug/ml](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Immunohistochemistry-Paraffin-NBP1-76767-img0002.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22069_rabbit-april-tnfsf13-mab-sr1578-5720231558489.jpg "Western Blot: APRIL/TNFSF13 Antibody (SR1578) [NBP3-22069] - Western blot analysis using NBP3-22069 in Jurkat cell lysate.")
![Western Blot: APRIL/TNFSF13 Overexpression Lysate [NBL1-17164] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13.](http://images.novusbio.com/fullsize/TNFSF13-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17164-img0002.jpg "Western Blot: APRIL/TNFSF13 Overexpression Lysate [NBL1-17164] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13.")
![Western Blot: APRIL/TNFSF13 Overexpression Lysate [NBL1-17165] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13.](http://images.novusbio.com/fullsize/TNFSF13-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17165-img0002.jpg "Western Blot: APRIL/TNFSF13 Overexpression Lysate [NBL1-17165] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13.")