| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51646-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14116 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08320 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08344 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21079 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21080 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24008 | |||
![Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/SMAD4-Antibody-Western-Blot-NBP2-24951-img0001.jpg "Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg)
![Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Western-Blot-NBP3-15673-img0004.jpg "Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (1H7D3) [NBP3-15673] - Human liver cancer using [KO Validated] Smad4 Rabbit mAb (NBP3-15673) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Immunohistochemistry-Paraffin-NBP3-15673-img0002.jpg)
![Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Western-Blot-NBP3-15673-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Smad4 Antibody (1H7D3) [NBP3-15673] - Western blot analysis of extracts from normal (control) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Knockout-Validated-NBP3-15673-img0001.jpg)
![Immunoprecipitation: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of 300ug extracts of 293T cells using 3ug Smad4 antibody (NBP3-15673). Western blot was performed from the immunoprecipitate using Smad4 antibody (NBP3-15673) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Immunoprecipitation-NBP3-15673-img0005.jpg)
![Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Western-Blot-NBP3-05507-img0006.jpg "Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human placenta shows moderate nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human prostate shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human stomach cancer shows strong cytoplasmic and nuclear positivity.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0004.jpg)
![Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Western-Blot-NBP1-51646-img0015.jpg "Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunocytochemistry-NBP1-51646-img0012.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of lung cancer tissues using SMAD4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunohistochemistry-Paraffin-NBP1-51646-img0014.jpg)
![Flow Cytometry: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Flow-Cytometry-NBP1-51646-img0009.jpg)
![Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.](http://images.novusbio.com/fullsize/Smad4-Antibody-RM277-Western-Blot-NBP2-61587-img0003.jpg "Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61587.](http://images.novusbio.com/fullsize/Smad4-Antibody-RM277-Immunohistochemistry-Paraffin-NBP2-61587-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using NBP2-61587 at a 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp2-61587_rabbit-monoclonal-smad4-antibody-rm277-161020231947103.png)
![Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunoprecipitation-NBP3-14714-img0001.jpg "Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0001.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (Smad4/2440).](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD4-2440-Immunohistochemistry-Paraffin-NBP3-07262-img0002.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (Smad4/2440).")
![Protein Array: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Analysis of Protein Array containing >19,000 full-length human proteins using Smad4 Mouse Monoclonal Antibody (Smad4/2440).](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD4-2440-Protein-Array-NBP3-07262-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (SPM448) [NBP3-07263] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (SPM448).](http://images.novusbio.com/fullsize/Smad4-Antibody-SPM448-Immunohistochemistry-Paraffin-NBP3-07263-img0001.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SPM448) [NBP3-07263] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (SPM448).")
