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![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0025.jpg "Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Immunocytochemistry-Immunofluorescence-H00004086-M03-img0018.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Immunohistochemistry-Paraffin-H00004086-M03-img0020.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0021.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0022.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in IMR-32 (Cat # L008V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0023.jpg)
![Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-Western-Blot-H00004086-M03-img0024.jpg)
![ELISA: Smad1 Antibody (2E9) [H00004086-M03] - Detection limit for recombinant GST tagged SMAD1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad1-Antibody-2E9-ELISA-H00004086-M03-img0009.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.Lane 1: SMAD1 transfected lysate(52.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0023.jpg "Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.Lane 1: SMAD1 transfected lysate(52.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Immunocytochemistry-Immunofluorescence-H00004086-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (1D3) [H00004086-M02] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Immunohistochemistry-Paraffin-H00004086-M02-img0018.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3. Analysis of SMAD1 expression in A-549.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0020.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3 Analysis of SMAD1 expression in IMR-32.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0021.jpg)
![Western Blot: Smad1 Antibody (1D3) [H00004086-M02] - SMAD1 monoclonal antibody (M02), clone 1D3. Analysis of SMAD1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Western-Blot-H00004086-M02-img0022.jpg)
![Sandwich ELISA: Smad1 Antibody (1D3) [H00004086-M02] - Detection limit for recombinant GST tagged SMAD1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad1-Antibody-1D3-Sandwich-ELISA-H00004086-M02-img0019.jpg)
![Western Blot: SMAD1/5/8/9 Antibody [NB100-56656] - Analysis using SMAD1/5/8/9 antibody. Lysate from Saos-2 cells probed with SMAD antibody.](http://images.novusbio.com/fullsize/SMAD1-5-8-9-Antibody-Western-Blot-NB100-56656-img0003.jpg "Western Blot: SMAD1/5/8/9 Antibody [NB100-56656] - Analysis using SMAD1/5/8/9 antibody. Lysate from Saos-2 cells probed with SMAD antibody.")
![Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Western-Blot-NBP3-15670-img0006.jpg "Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad1 Rabbit mAb (NBP3-15670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunocytochemistry-Immunofluorescence-NBP3-15670-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0004.jpg)
![Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of various cell lines, using Smad1 antibody (NBP3-15670) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Western-Blot-NBP3-15670-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded mouse kidney using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Immunohistochemistry-Paraffin-NBP3-15670-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts from normal (control) and Smad1 knockout (KO) HeLa cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Smad1-Antibody-4W2M9-Knockout-Validated-NBP3-15670-img0001.jpg)
![Western Blot: SMAD1/5/8/9 Antibody [NB600-962] - Analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/SMAD1-5-8-9-Antibody-Western-Blot-NB600-962-img0002.jpg "Western Blot: SMAD1/5/8/9 Antibody [NB600-962] - Analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0017.jpg "Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Immunocytochemistry-Immunofluorescence-H00004086-M04-img0012.jpg)
![Immunohistochemistry-Paraffin: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Immunohistochemistry-Paraffin-H00004086-M04-img0014.jpg)
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0015.jpg)
![Western Blot: Smad1 Antibody (2A1) [H00004086-M04] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-2A1-Western-Blot-H00004086-M04-img0016.jpg)
![Western Blot: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Western-Blot-NBP2-45869-img0004.jpg "Western Blot: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1.")
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0003.jpg)
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0001.jpg)
![Immunohistochemistry: Smad1 Antibody (1C5) [NBP2-45869] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Smad1-Antibody-1C5-Immunohistochemistry-NBP2-45869-img0002.jpg)
![Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SMAD1-9-Antibody-4E12H10-Western-Blot-NBP2-61878-img0003.jpg "Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mAb against human SMAD1 (AA: 1-110) recombinant protein. (Expected MW is 38.5 kDa)](http://images.novusbio.com/fullsize/SMAD1-9-Antibody-4E12H10-Western-Blot-NBP2-61878-img0002.jpg)
![Western Blot: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Analysis using SMAD1 mouse mAb against NIH/3T3 (1), COS7 (2), HUVEC (3), and C2C12 (4) cell lysate.](http://images.novusbio.com/fullsize/SMAD1-9-Antibody-4E12H10-Western-Blot-NBP2-61878-img0004.jpg)
![ELISA: SMAD1/9 Antibody (4E12H10) [NBP2-61878] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SMAD1-9-Antibody-4E12H10-ELISA-NBP2-61878-img0001.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0008.jpg "Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0005.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0006.jpg)
![Western Blot: Smad1 Antibody (1B8) [H00004086-M06] - Analysis of SMAD1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Smad1-Antibody-1B8-Western-Blot-H00004086-M06-img0007.jpg)
![Recombinant Human Smad1 Protein [H00004086-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004086-P01-1.jpg "Recombinant Human Smad1 Protein [H00004086-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.](http://images.novusbio.com/fullsize/nbp3-18834_rabbit-smad1-5-9-mab-rm487-161120231301814.png "Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.")