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![Western Blot: PHPT1 Antibody [NBP2-94440] - Western blot analysis of extracts of various cell lines, using PHPT1 Rabbit pAb (NBP2-94440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/PHPT1 Antibody-Western Blot-NBP2-94440-img0004.jpg "Western Blot: PHPT1 Antibody [NBP2-94440] - Western blot analysis of extracts of various cell lines, using PHPT1 Rabbit pAb (NBP2-94440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-94440] - Paraffin-embedded mouse kidney using PHPT1 .](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-94440-img0002.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-94440] - Paraffin-embedded mouse heart using PHPT1 .](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-94440-img0001.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-94440] - Paraffin-embedded rat lung using PHPT1 .](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-94440-img0003.jpg)
![Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Western-Blot-NBP2-99999-img0005.jpg "Western Blot: PHPT1 Antibody [NBP2-99999] - Lane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa")
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP2-99999] - Staining of human PHPT1 in human skeletal muscle with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP2-99999-img0003.jpg)
![Immunoprecipitation: PHPT1 Antibody [NBP2-99999] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PHPT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PHPT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunoprecipitation-NBP2-99999-img0004.jpg)
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.](http://images.novusbio.com/fullsize/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88804-img0006.jpg "Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.")
![Immunocytochemistry/Immunofluorescence: PHPT1 Antibody (001) [NBP2-90108] - Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-90108-img0003.jpg "Immunocytochemistry/Immunofluorescence: PHPT1 Antibody (001) [NBP2-90108] - Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunohistochemistry-Paraffin: PHPT1 Antibody (001) [NBP2-90108] - Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90108-img0001.jpg)
![Flow Cytometry: PHPT1 Antibody (001) [NBP2-90108] - Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/PHPT1-Antibody-001-Flow-Cytometry-NBP2-90108-img0002.jpg)
![Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - Anti-PHPT1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: THP1 Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa. Observed band size:14 kDa.](http://images.novusbio.com/fullsize/PHPT1-Antibody-007-Immunoprecipitation-NBP2-90109-img0003.jpg "Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - Anti-PHPT1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: THP1 Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa. Observed band size:14 kDa.")
![Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - PHPT1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-PHPT1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PHPT1 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size :14 kDa.](http://images.novusbio.com/fullsize/PHPT1-Antibody-007-Immunoprecipitation-NBP2-90109-img0004.jpg)
![SDS-Page: Recombinant Human PHPT1 Protein [NBP2-59581] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-PHPT1-Protein-SDS-Page-NBP2-59581-img0001.jpg "SDS-Page: Recombinant Human PHPT1 Protein [NBP2-59581] - 15% SDS-PAGE (3ug)")
![Western Blot: PHPT1 Overexpression Lysate (Adult Normal) [NBL1-14372] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHPT1.](http://images.novusbio.com/fullsize/PHPT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14372-img0002.jpg "Western Blot: PHPT1 Overexpression Lysate (Adult Normal) [NBL1-14372] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHPT1.")
![Western Blot: PHPT1 Antibody [NBP2-88050] - WB Suggested Anti-Phpt1 Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Brain](http://images.novusbio.com/fullsize/PHPT1-Antibody-Western-Blot-NBP2-88050-img0001.jpg "Western Blot: PHPT1 Antibody [NBP2-88050] - WB Suggested Anti-Phpt1 Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Brain")
![Western Blot: PHPT1 Antibody [NBP2-94537] - Analysis of extracts of various cell lines, using PHPT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/PHPT1-Antibody-Western-Blot-NBP2-94537-img0001.jpg "Western Blot: PHPT1 Antibody [NBP2-94537] - Analysis of extracts of various cell lines, using PHPT1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![SDS-Page: Recombinant Human PHPT1 Protein [NBP1-49439] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-PHPT1-Protein-SDS-Page-NBP1-49439-img0001.jpg "SDS-Page: Recombinant Human PHPT1 Protein [NBP1-49439] - 15 % SDS-PAGE (3ug)")