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![Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1/NLGN1 protein using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-S97A-31-Western-Blot-NBP2-42192-img0003.jpg "Western Blot: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Western Blot analysis of Mouse Brain Membrane showing detection of ~120 kDa Neuroligin 1/NLGN1 protein using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~120 kDa.")
![Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1/NLGN1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-S97A-31-Immunocytochemistry-Immunofluorescence-NBP2-42192-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-S97A-31-Immunocytochemistry-Immunofluorescence-NBP2-42192-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody (S97A-31) [NBP2-42192] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody, Clone S97A-31 (NBP2-42192). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 1/NLGN1 Monoclonal Antibody (NBP2-42192) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 1/NLGN1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-S97A-31-Immunocytochemistry-Immunofluorescence-NBP2-42192-img0005.jpg)
![Western Blot: Neuroligin 1/NLGN1 Antibody [NBP1-87888] - Analysis in control (vector only transfected HEK293T lysate) and NLGN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Western-Blot-NBP1-87888-img0006.jpg "Western Blot: Neuroligin 1/NLGN1 Antibody [NBP1-87888] - Analysis in control (vector only transfected HEK293T lysate) and NLGN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Neuroligin 1/NLGN1 Antibody [NBP1-87888] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87888-img0005.jpg)
![Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Immunofluorescence-NBP2-76348-img0001.jpg "Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Immunofluorescence-NBP2-76348-img0002.jpg)
![Western Blot: Neuroligin 1/NLGN1 Antibody [NBP3-13175] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuroligin 1/NLGN1 antibody (NBP3-13175) diluted at 1:1000.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Western-Blot-NBP3-13175-img0001.jpg "Western Blot: Neuroligin 1/NLGN1 Antibody [NBP3-13175] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuroligin 1/NLGN1 antibody (NBP3-13175) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP3-13175] - Neuroligin 1/NLGN1 antibody detects Neuroligin 1/NLGN1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuroligin 1/NLGN1 protein stained by Neuroligin 1/NLGN1 antibody (NBP3-13175) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13175-img0002.jpg)
![Western Blot: Neuroligin 1/NLGN1 Antibody [NBP2-19520] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Neuroligin-1-NLGN1-Antibody-Western-Blot-NBP2-19520-img0002.jpg "Western Blot: Neuroligin 1/NLGN1 Antibody [NBP2-19520] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.")