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![Western Blot: Neogenin Antibody [NBP1-89651] - Analysis in human cell lines A-549 and HeLa using anti-NEO1 antibody. Corresponding NEO1 RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Western-Blot-NBP1-89651-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Neogenin Antibody [NBP1-89651] - Analysis in human cell lines A-549 and HeLa using anti-NEO1 antibody. Corresponding NEO1 RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Neogenin Antibody [NBP1-89651] - Staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89651-img0007.jpg)
![Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89651-img0017.jpg)
![Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89651-img0014.jpg)
![Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89651-img0015.jpg)
![Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89651-img0016.jpg)
![Western Blot: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Western-Blot-NBP2-32272-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Neogenin Antibody [NBP2-32272] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody NBP2-32272 used for IP at 6 ug per reaction. Neogenin was also immunoprecipitated by rabbit anti-Neogenin antibody BL16480. For blotting immunoprecipitated Neogenin, NBP2-32272 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunoprecipitation-NBP2-32272-img0002.jpg)
![Immunohistochemistry: Neogenin Antibody [NBP1-89653] - Staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-NBP1-89653-img0003.jpg "Immunohistochemistry: Neogenin Antibody [NBP1-89653] - Staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.")
![Immunohistochemistry: Neogenin Antibody [NBP1-89652] - Staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Neogenin-Antibody-Immunohistochemistry-NBP1-89652-img0003.jpg "Immunohistochemistry: Neogenin Antibody [NBP1-89652] - Staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry: Neogenin Antibody (RM0124-3G55) [NBP1-22501]](http://images.novusbio.com/fullsize/Neogenin-Antibody-RM0124-3G55-Immunohistochemistry-NBP1-22501-img0001.jpg "Immunohistochemistry: Neogenin Antibody (RM0124-3G55) [NBP1-22501]")
![Western Blot: Neogenin Overexpression Lysate (Adult Normal) [NBL1-13591] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Neogenin.](http://images.novusbio.com/fullsize/Neogenin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13591-img0002.jpg "Western Blot: Neogenin Overexpression Lysate (Adult Normal) [NBL1-13591] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Neogenin.")
![ELISA: Human Neogenin ELISA Kit (Colorimetric) [NBP3-08158] - Samples were spiked with high concentrations of Human Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08158_human-neogenin-elisa-kit-colorimetric-132202416201945.jpg "ELISA: Human Neogenin ELISA Kit (Colorimetric) [NBP3-08158] - Samples were spiked with high concentrations of Human Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Neogenin ELISA Kit (Colorimetric) [NBP3-08158] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08158_human-neogenin-elisa-kit-colorimetric-432024857415.jpg)
![ELISA: Mouse Neogenin ELISA Kit (Colorimetric) [NBP3-08157] - Samples were spiked with high concentrations of Mouse Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08157_mouse-neogenin-elisa-kit-colorimetric-13220241620470.jpg "ELISA: Mouse Neogenin ELISA Kit (Colorimetric) [NBP3-08157] - Samples were spiked with high concentrations of Mouse Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Neogenin ELISA Kit (Colorimetric) [NBP3-08157] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08157_mouse-neogenin-elisa-kit-colorimetric-432024941560.jpg)
![ELISA: Rat Neogenin ELISA Kit (Colorimetric) [NBP3-08159] - Samples were spiked with high concentrations of Rat Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08159_rat-neogenin-elisa-kit-colorimetric-132202416232372.jpg "ELISA: Rat Neogenin ELISA Kit (Colorimetric) [NBP3-08159] - Samples were spiked with high concentrations of Rat Neogenin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Neogenin ELISA Kit (Colorimetric) [NBP3-08159] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08159_rat-neogenin-elisa-kit-colorimetric-432024955094.jpg)